TY - JOUR
T1 - Complete genome sequence of a novel endornavirus isolated from hot pepper
AU - Lim, Seungmo
AU - Kim, Kil Hyun
AU - Zhao, Fumei
AU - Yoo, Ran Hee
AU - Igori, Davaajargal
AU - Lee, Su Heon
AU - Moon, Jae Sun
N1 - Publisher Copyright:
© 2015, Springer-Verlag Wien.
PY - 2015/9/30
Y1 - 2015/9/30
N2 - The complete genome of a putative new endornavirus infecting hot peppers (Capsicum annuum) was determined to be 14,729 nt in size, including 12 cytosines at the 3′ end. The hot pepper-infecting virus has the highest nucleotide sequence similarity (94 % query cover and 72 % identity) to bell pepper endornavirus (BPEV) isolated from the cultivar Yolo Wonder in the USA (GenBank accession no. JN019858). The putative single, large open reading frame encodes a 4,884-amino-acid-long polyprotein that contains four putative functional domains: a viral methyltransferase, a viral RNA helicase, a glycosyltransferase, and an RNA-dependent RNA polymerase. A phylogenetic tree based on whole polyprotein sequences confirmed the close evolutionary relationship of the studied endornavirus to BPEV. The hot pepper-infecting virus also has a nick at nt position 975. Taken together, these results suggest that this virus belongs to a new species in the genus Endornavirus (family Endornaviridae), for which the name hot pepper endornavirus (HPEV) is proposed.
AB - The complete genome of a putative new endornavirus infecting hot peppers (Capsicum annuum) was determined to be 14,729 nt in size, including 12 cytosines at the 3′ end. The hot pepper-infecting virus has the highest nucleotide sequence similarity (94 % query cover and 72 % identity) to bell pepper endornavirus (BPEV) isolated from the cultivar Yolo Wonder in the USA (GenBank accession no. JN019858). The putative single, large open reading frame encodes a 4,884-amino-acid-long polyprotein that contains four putative functional domains: a viral methyltransferase, a viral RNA helicase, a glycosyltransferase, and an RNA-dependent RNA polymerase. A phylogenetic tree based on whole polyprotein sequences confirmed the close evolutionary relationship of the studied endornavirus to BPEV. The hot pepper-infecting virus also has a nick at nt position 975. Taken together, these results suggest that this virus belongs to a new species in the genus Endornavirus (family Endornaviridae), for which the name hot pepper endornavirus (HPEV) is proposed.
UR - http://www.scopus.com/inward/record.url?scp=84946494269&partnerID=8YFLogxK
U2 - 10.1007/s00705-015-2616-7
DO - 10.1007/s00705-015-2616-7
M3 - Article
C2 - 26424198
AN - SCOPUS:84946494269
SN - 0304-8608
VL - 160
SP - 3153
EP - 3156
JO - Archives of Virology
JF - Archives of Virology
IS - 12
ER -