TY - JOUR
T1 - Complete genome sequence of Deinococcus swuensis, a bacterium resistant to radiation toxicity
AU - Kim, Myung Kyum
AU - Srinivasan, Sathiyaraj
AU - Back, Chang Gi
AU - Joo, Eun Sun
AU - Lee, Seung Yeol
AU - Jung, Hee Young
N1 - Publisher Copyright:
© 2015, The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht.
PY - 2015/9/3
Y1 - 2015/9/3
N2 - Deinococcus swuensis DY59T is a Grampositive, coccus-shaped bacterium. Most members of the genus Deinococcus are able to grow in the presence of high levels of chronic radiation toxicity and desiccation because they can protect enzymes from reactive oxygen species generated during ionizing radiation. The mechanisms behind the resistance to radiation toxicity and the genomic features of resistance could be useful to exploit Deinococcus swuensis in the biotechnological applications such as detoxification of xenobiotic contaminated with radioactive wastes. Strain DY59T showed resistance to gamma radiation with a D10 value (i.e. the dose required to reduce the bacterial population by 10-fold) in excess of 5 kGy. However, the genus Deinococcus is slightly characterized at the genome level, despite its potential importance. Thus, the present study determined the features of Deinococcus swuensis DY59T, as well as its genome sequence and annotation. The genome comprised of 3,531,443 bp with a G + C content of 67.4%, which included 3,305 protein-coding genes and 58 RNA genes. Based on the genome annotation, the strain DY59T undergoes prokaryotic type nucleotide excision repair pathway, restores the damaged gene, and resists the ionizing radiation toxicity.
AB - Deinococcus swuensis DY59T is a Grampositive, coccus-shaped bacterium. Most members of the genus Deinococcus are able to grow in the presence of high levels of chronic radiation toxicity and desiccation because they can protect enzymes from reactive oxygen species generated during ionizing radiation. The mechanisms behind the resistance to radiation toxicity and the genomic features of resistance could be useful to exploit Deinococcus swuensis in the biotechnological applications such as detoxification of xenobiotic contaminated with radioactive wastes. Strain DY59T showed resistance to gamma radiation with a D10 value (i.e. the dose required to reduce the bacterial population by 10-fold) in excess of 5 kGy. However, the genus Deinococcus is slightly characterized at the genome level, despite its potential importance. Thus, the present study determined the features of Deinococcus swuensis DY59T, as well as its genome sequence and annotation. The genome comprised of 3,531,443 bp with a G + C content of 67.4%, which included 3,305 protein-coding genes and 58 RNA genes. Based on the genome annotation, the strain DY59T undergoes prokaryotic type nucleotide excision repair pathway, restores the damaged gene, and resists the ionizing radiation toxicity.
KW - Deinococcaceae
KW - Deinococcus swuensis
KW - Radiation resistance
UR - http://www.scopus.com/inward/record.url?scp=84942851382&partnerID=8YFLogxK
U2 - 10.1007/s13273-015-0031-5
DO - 10.1007/s13273-015-0031-5
M3 - Article
AN - SCOPUS:84942851382
SN - 1738-642X
VL - 11
SP - 315
EP - 321
JO - Molecular and Cellular Toxicology
JF - Molecular and Cellular Toxicology
IS - 3
ER -