Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

C. Nakamura; M. M. Liu; Y. K. Goo; G. H. Zhang; H. L. Jia; A. Kumagai; H. S. Zhang; J. L. Zhou; Y. Nishikawa; X. N. Xuan

doi:10.47665/TB.37.4.1029

Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

C. Nakamura, M. M. Liu, Y. K. Goo, G. H. Zhang, H. L. Jia, A. Kumagai, H. S. Zhang, J. L. Zhou, Y. Nishikawa, X. N. Xuan

Department of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/ BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.

Original language	English
Pages (from-to)	1029-1037
Number of pages	9
Journal	Tropical Biomedicine
Volume	37
Issue number	4
DOIs	https://doi.org/10.47665/TB.37.4.1029
State	Published - 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.47665/TB.37.4.1029

Cite this

Nakamura, C., Liu, M. M., Goo, Y. K., Zhang, G. H., Jia, H. L., Kumagai, A., Zhang, H. S., Zhou, J. L., Nishikawa, Y., & Xuan, X. N. (2020). Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice. Tropical Biomedicine, 37(4), 1029-1037. https://doi.org/10.47665/TB.37.4.1029

@article{7d51fae990c54945b369dca1ad3394d1,

title = "Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice",

abstract = "Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/ BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.",

author = "C. Nakamura and Liu, {M. M.} and Goo, {Y. K.} and Zhang, {G. H.} and Jia, {H. L.} and A. Kumagai and Zhang, {H. S.} and Zhou, {J. L.} and Y. Nishikawa and Xuan, {X. N.}",

year = "2020",

doi = "10.47665/TB.37.4.1029",

language = "English",

volume = "37",

pages = "1029--1037",

journal = "Tropical Biomedicine",

issn = "0127-5720",

publisher = "Malaysian Society of Parasitology and Tropical Medicine",

number = "4",

}

TY - JOUR

T1 - Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

AU - Nakamura, C.

AU - Liu, M. M.

AU - Goo, Y. K.

AU - Zhang, G. H.

AU - Jia, H. L.

AU - Kumagai, A.

AU - Zhang, H. S.

AU - Zhou, J. L.

AU - Nishikawa, Y.

AU - Xuan, X. N.

PY - 2020

Y1 - 2020

N2 - Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/ BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.

AB - Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/ BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.

UR - http://www.scopus.com/inward/record.url?scp=85099815345&partnerID=8YFLogxK

U2 - 10.47665/TB.37.4.1029

DO - 10.47665/TB.37.4.1029

M3 - Article

C2 - 33612755

AN - SCOPUS:85099815345

SN - 0127-5720

VL - 37

SP - 1029

EP - 1037

JO - Tropical Biomedicine

JF - Tropical Biomedicine

IS - 4

ER -

Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this