Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

C. Nakamura; M. M. Liu; Y. K. Goo; G. H. Zhang; H. L. Jia; A. Kumagai; H. S. Zhang; J. L. Zhou; Y. Nishikawa; X. N. Xuan

doi:10.47665/TB.37.4.1029

Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

C. Nakamura
, M. M. Liu
, Y. K. Goo
, G. H. Zhang
, H. L. Jia
, A. Kumagai
, H. S. Zhang
, J. L. Zhou
, Y. Nishikawa
, X. N. Xuan

Department of Medicine

Obihiro University of Agriculture and Veterinary Medicine
Chinese Academy of Agricultural Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/ BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.

Original language	English
Pages (from-to)	1029-1037
Number of pages	9
Journal	Tropical Biomedicine
Volume	37
Issue number	4
DOIs	https://doi.org/10.47665/TB.37.4.1029
State	Published - 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.47665/TB.37.4.1029

Cite this

Nakamura, C., Liu, M. M., Goo, Y. K., Zhang, G. H., Jia, H. L., Kumagai, A., Zhang, H. S., Zhou, J. L., Nishikawa, Y., & Xuan, X. N. (2020). Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice. Tropical Biomedicine, 37(4), 1029-1037. https://doi.org/10.47665/TB.37.4.1029

@article{7d51fae990c54945b369dca1ad3394d1,

title = "Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice",

abstract = "Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/ BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.",

author = "C. Nakamura and Liu, \{M. M.\} and Goo, \{Y. K.\} and Zhang, \{G. H.\} and Jia, \{H. L.\} and A. Kumagai and Zhang, \{H. S.\} and Zhou, \{J. L.\} and Y. Nishikawa and Xuan, \{X. N.\}",

year = "2020",

doi = "10.47665/TB.37.4.1029",

language = "English",

volume = "37",

pages = "1029--1037",

journal = "Tropical Biomedicine",

issn = "0127-5720",

publisher = "Malaysian Society for Parasitology",

number = "4",

}

TY - JOUR

T1 - Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

AU - Nakamura, C.

AU - Liu, M. M.

AU - Goo, Y. K.

AU - Zhang, G. H.

AU - Jia, H. L.

AU - Kumagai, A.

AU - Zhang, H. S.

AU - Zhou, J. L.

AU - Nishikawa, Y.

AU - Xuan, X. N.

PY - 2020

Y1 - 2020

N2 - Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/ BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.

AB - Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/ BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific antiBgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.

UR - https://www.scopus.com/pages/publications/85099815345

U2 - 10.47665/TB.37.4.1029

DO - 10.47665/TB.37.4.1029

M3 - Article

C2 - 33612755

AN - SCOPUS:85099815345

SN - 0127-5720

VL - 37

SP - 1029

EP - 1037

JO - Tropical Biomedicine

JF - Tropical Biomedicine

IS - 4

ER -

Construction of a recombinant vaccinia virus expressing babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this