Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice

Guohong Zhang; Xiaohong Huang; Damdinsuren Boldbaatar; Banzragch Battur; Badgar Battsetseg; Houshuang Zhang; Longzheng Yu; Yan Li; Yuzi Luo; Shinuo Cao; Youn Kyong Goo; Junya Yamagishi; Jinlin Zhou; Shoufa Zhang; Hiroshi Suzuki; Ikuo Igarashi; Takeshi Mikami; Yoshifumi Nishikawa; Xuenan Xuan

doi:10.1016/j.vaccine.2010.08.096

Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice

Guohong Zhang, Xiaohong Huang, Damdinsuren Boldbaatar, Banzragch Battur, Badgar Battsetseg, Houshuang Zhang, Longzheng Yu, Yan Li, Yuzi Luo, Shinuo Cao, Youn Kyong Goo, Junya Yamagishi, Jinlin Zhou, Shoufa Zhang, Hiroshi Suzuki, Ikuo Igarashi, Takeshi Mikami, Yoshifumi Nishikawa, Xuenan Xuan

Department of Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine.

Original language	English
Pages (from-to)	7243-7247
Number of pages	5
Journal	Vaccine
Volume	28
Issue number	45
DOIs	https://doi.org/10.1016/j.vaccine.2010.08.096
State	Published - 21 Oct 2010

Keywords

Neospora caninum
Recombinant vaccine
TgSAG1
Toxoplasma gondii
Vector

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2010.08.096

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Zhang, G., Huang, X., Boldbaatar, D., Battur, B., Battsetseg, B., Zhang, H., Yu, L., Li, Y., Luo, Y., Cao, S., Goo, Y. K., Yamagishi, J., Zhou, J., Zhang, S., Suzuki, H., Igarashi, I., Mikami, T., Nishikawa, Y., & Xuan, X. (2010). Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice. Vaccine, 28(45), 7243-7247. https://doi.org/10.1016/j.vaccine.2010.08.096

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title = "Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice",

abstract = "Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine.",

keywords = "Neospora caninum, Recombinant vaccine, TgSAG1, Toxoplasma gondii, Vector",

author = "Guohong Zhang and Xiaohong Huang and Damdinsuren Boldbaatar and Banzragch Battur and Badgar Battsetseg and Houshuang Zhang and Longzheng Yu and Yan Li and Yuzi Luo and Shinuo Cao and Goo, {Youn Kyong} and Junya Yamagishi and Jinlin Zhou and Shoufa Zhang and Hiroshi Suzuki and Ikuo Igarashi and Takeshi Mikami and Yoshifumi Nishikawa and Xuenan Xuan",

year = "2010",

month = oct,

day = "21",

doi = "10.1016/j.vaccine.2010.08.096",

language = "English",

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Zhang, G, Huang, X, Boldbaatar, D, Battur, B, Battsetseg, B, Zhang, H, Yu, L, Li, Y, Luo, Y, Cao, S, Goo, YK, Yamagishi, J, Zhou, J, Zhang, S, Suzuki, H, Igarashi, I, Mikami, T, Nishikawa, Y & Xuan, X 2010, 'Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice', Vaccine, vol. 28, no. 45, pp. 7243-7247. https://doi.org/10.1016/j.vaccine.2010.08.096

TY - JOUR

T1 - Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice

AU - Zhang, Guohong

AU - Huang, Xiaohong

AU - Boldbaatar, Damdinsuren

AU - Battur, Banzragch

AU - Battsetseg, Badgar

AU - Zhang, Houshuang

AU - Yu, Longzheng

AU - Li, Yan

AU - Luo, Yuzi

AU - Cao, Shinuo

AU - Goo, Youn Kyong

AU - Yamagishi, Junya

AU - Zhou, Jinlin

AU - Zhang, Shoufa

AU - Suzuki, Hiroshi

AU - Igarashi, Ikuo

AU - Mikami, Takeshi

AU - Nishikawa, Yoshifumi

AU - Xuan, Xuenan

PY - 2010/10/21

Y1 - 2010/10/21

N2 - Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine.

AB - Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine.

KW - Neospora caninum

KW - Recombinant vaccine

KW - TgSAG1

KW - Toxoplasma gondii

KW - Vector

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U2 - 10.1016/j.vaccine.2010.08.096

DO - 10.1016/j.vaccine.2010.08.096

M3 - Article

C2 - 20832493

AN - SCOPUS:77957753779

SN - 0264-410X

VL - 28

SP - 7243

EP - 7247

JO - Vaccine

JF - Vaccine

IS - 45

ER -

Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice

Abstract

Keywords

UN SDGs

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