Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

Mi Ja Park; Seong Joon Joh; Kang Seuk Choi; Aeran Kim; Min Goo Seo; Jae Young Song; Seon Jong Yun

doi:10.14405/kjvr.2016.56.3.189

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

Mi Ja Park, Seong Joon Joh, Kang Seuk Choi, Aeran Kim, Min Goo Seo, Jae Young Song, Seon Jong Yun

Department of Veterinary Medicine

Animal and Plant Quarantine Agency

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log₁₀ and HI titer of 5 log₂ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

Original language	English
Pages (from-to)	188-192
Number of pages	5
Journal	Korean Journal of Veterinary Research
Volume	56
Issue number	3
DOIs	https://doi.org/10.14405/kjvr.2016.56.3.189
State	Published - 2016

Keywords

Hemagglutination inhibition
Infectious bronchitis
Massachusetts 41 vaccine
Potency test

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.14405/kjvr.2016.56.3.189

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Park, M. J., Joh, S. J., Choi, K. S., Kim, A., Seo, M. G., Song, J. Y., & Yun, S. J. (2016). Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency. Korean Journal of Veterinary Research, 56(3), 188-192. https://doi.org/10.14405/kjvr.2016.56.3.189

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title = "Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency",

abstract = "The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r2 values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log10 and HI titer of 5 log2 was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.",

keywords = "Hemagglutination inhibition, Infectious bronchitis, Massachusetts 41 vaccine, Potency test",

author = "Park, {Mi Ja} and Joh, {Seong Joon} and Choi, {Kang Seuk} and Aeran Kim and Seo, {Min Goo} and Song, {Jae Young} and Yun, {Seon Jong}",

year = "2016",

doi = "10.14405/kjvr.2016.56.3.189",

language = "English",

volume = "56",

pages = "188--192",

journal = "Korean Journal of Veterinary Research",

issn = "2466-1384",

publisher = "Korean Society of Veterinary Science",

number = "3",

}

Park, MJ, Joh, SJ, Choi, KS, Kim, A, Seo, MG, Song, JY & Yun, SJ 2016, 'Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency', Korean Journal of Veterinary Research, vol. 56, no. 3, pp. 188-192. https://doi.org/10.14405/kjvr.2016.56.3.189

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency. / Park, Mi Ja; Joh, Seong Joon; Choi, Kang Seuk et al.
In: Korean Journal of Veterinary Research, Vol. 56, No. 3, 2016, p. 188-192.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

AU - Park, Mi Ja

AU - Joh, Seong Joon

AU - Choi, Kang Seuk

AU - Kim, Aeran

AU - Seo, Min Goo

AU - Song, Jae Young

AU - Yun, Seon Jong

PY - 2016

Y1 - 2016

N2 - The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r2 values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log10 and HI titer of 5 log2 was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

AB - The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r2 values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log10 and HI titer of 5 log2 was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

KW - Hemagglutination inhibition

KW - Infectious bronchitis

KW - Massachusetts 41 vaccine

KW - Potency test

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DO - 10.14405/kjvr.2016.56.3.189

M3 - Article

AN - SCOPUS:84989211568

SN - 2466-1384

VL - 56

SP - 188

EP - 192

JO - Korean Journal of Veterinary Research

JF - Korean Journal of Veterinary Research

IS - 3

ER -

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

Abstract

Keywords

UN SDGs

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