Critical diagnostic and cancer stem cell markers in neoplastic cells from canine primary and xenografted pulmonary adenocarcinoma

Warisraporn Tangchang; Yun Hyeok Kim; Ye In Oh; Byung Woo Lee; Hyunwook Kim; Byungil Yoon

doi:10.4142/jvs.22124

Critical diagnostic and cancer stem cell markers in neoplastic cells from canine primary and xenografted pulmonary adenocarcinoma

Warisraporn Tangchang
, Yun Hyeok Kim
, Ye In Oh
, Byung Woo Lee
, Hyunwook Kim
, Byungil Yoon

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

It is challenging to diagnose metastatic tumors whose cellular morphology is different from the primary. We characterized canine primary pulmonary adenocarcinoma (PAC) and its xenografted tumors by histological and immunohistochemical analyses for critical diagnostic and cancer stem cell (CSC) markers. To generate a tumor xenograft model, we subsequently transplanted the tissue pieces from the PAC into athymic nude mice. Immunohistochemical examination was performed for diagnostic (TTF-1, Napsin A, and SP-A) and CSC markers (CD44 and CD133). The use of CSC markers together with diagnostic markers can improve the detection and diagnosis of canine primary and metastatic adenocarcinomas.

Original language	English
Article number	e89
Journal	Journal of Veterinary Science
Volume	23
Issue number	6
DOIs	https://doi.org/10.4142/jvs.22124
State	Published - 2022

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SDG 3 Good Health and Well-being

Keywords

Canine pulmonary adenocarcinoma
Diagnostic markers
Immunohistochemistry
Stem cell markers
Tumor xenograft model

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10.4142/jvs.22124

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title = "Critical diagnostic and cancer stem cell markers in neoplastic cells from canine primary and xenografted pulmonary adenocarcinoma",

abstract = "It is challenging to diagnose metastatic tumors whose cellular morphology is different from the primary. We characterized canine primary pulmonary adenocarcinoma (PAC) and its xenografted tumors by histological and immunohistochemical analyses for critical diagnostic and cancer stem cell (CSC) markers. To generate a tumor xenograft model, we subsequently transplanted the tissue pieces from the PAC into athymic nude mice. Immunohistochemical examination was performed for diagnostic (TTF-1, Napsin A, and SP-A) and CSC markers (CD44 and CD133). The use of CSC markers together with diagnostic markers can improve the detection and diagnosis of canine primary and metastatic adenocarcinomas.",

keywords = "Canine pulmonary adenocarcinoma, Diagnostic markers, Immunohistochemistry, Stem cell markers, Tumor xenograft model",

author = "Warisraporn Tangchang and Kim, \{Yun Hyeok\} and Oh, \{Ye In\} and Lee, \{Byung Woo\} and Hyunwook Kim and Byungil Yoon",

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AU - Tangchang, Warisraporn

AU - Kim, Yun Hyeok

AU - Oh, Ye In

AU - Lee, Byung Woo

AU - Kim, Hyunwook

AU - Yoon, Byungil

N1 - Publisher Copyright: © 2022 The Korean Society of Veterinary Science This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2022

Y1 - 2022

N2 - It is challenging to diagnose metastatic tumors whose cellular morphology is different from the primary. We characterized canine primary pulmonary adenocarcinoma (PAC) and its xenografted tumors by histological and immunohistochemical analyses for critical diagnostic and cancer stem cell (CSC) markers. To generate a tumor xenograft model, we subsequently transplanted the tissue pieces from the PAC into athymic nude mice. Immunohistochemical examination was performed for diagnostic (TTF-1, Napsin A, and SP-A) and CSC markers (CD44 and CD133). The use of CSC markers together with diagnostic markers can improve the detection and diagnosis of canine primary and metastatic adenocarcinomas.

AB - It is challenging to diagnose metastatic tumors whose cellular morphology is different from the primary. We characterized canine primary pulmonary adenocarcinoma (PAC) and its xenografted tumors by histological and immunohistochemical analyses for critical diagnostic and cancer stem cell (CSC) markers. To generate a tumor xenograft model, we subsequently transplanted the tissue pieces from the PAC into athymic nude mice. Immunohistochemical examination was performed for diagnostic (TTF-1, Napsin A, and SP-A) and CSC markers (CD44 and CD133). The use of CSC markers together with diagnostic markers can improve the detection and diagnosis of canine primary and metastatic adenocarcinomas.

KW - Canine pulmonary adenocarcinoma

KW - Diagnostic markers

KW - Immunohistochemistry

KW - Stem cell markers

KW - Tumor xenograft model

UR - https://www.scopus.com/pages/publications/85143767637

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DO - 10.4142/jvs.22124

M3 - Article

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SN - 1229-845X

VL - 23

JO - Journal of Veterinary Science

JF - Journal of Veterinary Science

IS - 6

M1 - e89

ER -

Critical diagnostic and cancer stem cell markers in neoplastic cells from canine primary and xenografted pulmonary adenocarcinoma

Abstract

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Keywords

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