Abstract
The objective of this study was to investigate the effect of glycerol on the cryopreservation fin expiants of goldfish, Carassius auratus. Four different concentrations, 5, 10, 15, and 20% (v/v) of glycerol and a control were tested. These were prepared in Dulbecco's modified Eagle's medium with 20% (v/v) Fetal Bovine Serum. Attachment and outgrowing rates were monitored from day 3 to day 14. Results showed that fin expiants cryopreserved in 20% concentration of glycerol was significantly higher (P<0.05) with a 100% attachment rate compared to 5, 10, and 15% concentrations with 36.67, 84.19 and 86.51% attachment rate, respectively. Fin expiants cryopreserved in 20% glycerol concentration also had significantly higher (P<0.05) outgrowth of cells (72.50%) than the other three concentrations on day 3. Moreover, a 100% outgrowth of cells in all concentrations was achieved after 14 days of culture. No attachment and out growth of cells were observed in control group. Goldfish caudal fin expiants cryopreserved in glycerol can produce live cells efficiently, regardless of concentration.
| Original language | English |
|---|---|
| Pages (from-to) | 57-61 |
| Number of pages | 5 |
| Journal | Cryo-Letters |
| Volume | 32 |
| Issue number | 1 |
| State | Published - Jan 2011 |
Keywords
- Caudal fin
- Cryopreservation
- Fish expiants
- Glycerol
- Goldfish