Crystal Structure and Regiospecificity of Catechol O-Methyltransferase from Niastella koreensis

Seul Hoo Lee, Bongsang Kim, Kyung Jin Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Catechol O-methyltransferase (COMT) is an enzyme that transfers a methyl group to the catechol-derivative substrates using S-adenosyl-l-methionine (SAM) and Mg2+. We report the biochemical and structural analysis of COMT from Niastella koreensis (NkCOMT). NkCOMT showed the highest activity with Mg2+, although the enzyme also showed a significant level of activity with Cu2+ and Zn2+. NkCOMT structures complexed with SAH and Mg2+ elucidated how the enzyme stabilized the cosubstrate and the metal ion and revealed that the region near the SAM binding site undergoes conformational changes upon the binding of the cosubstrate and the metal ion. We also identified the catechol binding pocket of the enzyme and explained a broad substrate specificity of the bacterial enzyme and its ability to accommodate the catechol derivatives. In addition, we developed the NkCOMTE211R and NkCOMTE211K variants that showed both enhanced activities and regiospecificity for the production of the para-forms. Our study provides a structural basis for regiospecificity of NkCOMT, which is related with the conformational change upon binding of SAM and Mg2+.

Original languageEnglish
Pages (from-to)2531-2538
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume69
Issue number8
DOIs
StatePublished - 3 Mar 2021

Keywords

  • catechol O-methyltransferase
  • Niastella koreensis
  • regiospecificity
  • S-adenosyl-l-methionine

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