Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis

Ha Yeon Cho; Hyo Je Cho; Young Min Kim; Jeong Il Oh; Beom Sik Kang

doi:10.1107/S1744309108005186

Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis

Ha Yeon Cho, Hyo Je Cho, Young Min Kim, Jeong Il Oh, Beom Sik Kang

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS-DevR is a two-component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting-drop vapour-diffusion method in the presence of sodium citrate and 2-propanol as precipitants. X-ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 Å on a synchrotron beamline. The crystals belong to the hexagonal space group P6₁. The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 Å³ Da^-1. The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress.

Original language	English
Pages (from-to)	274-276
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	64
Issue number	4
DOIs	https://doi.org/10.1107/S1744309108005186
State	Published - 29 Mar 2008

Keywords

GAF domain
Hypoxia sensing
Mycobacteria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1107/S1744309108005186

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title = "Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis",

abstract = "Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS-DevR is a two-component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting-drop vapour-diffusion method in the presence of sodium citrate and 2-propanol as precipitants. X-ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 {\AA} on a synchrotron beamline. The crystals belong to the hexagonal space group P61. The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 {\AA}3 Da-1. The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress.",

keywords = "GAF domain, Hypoxia sensing, Mycobacteria",

author = "Cho, {Ha Yeon} and Cho, {Hyo Je} and Kim, {Young Min} and Oh, {Jeong Il} and Kang, {Beom Sik}",

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doi = "10.1107/S1744309108005186",

language = "English",

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pages = "274--276",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis. / Cho, Ha Yeon; Cho, Hyo Je; Kim, Young Min et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 64, No. 4, 29.03.2008, p. 274-276.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis

AU - Cho, Ha Yeon

AU - Cho, Hyo Je

AU - Kim, Young Min

AU - Oh, Jeong Il

AU - Kang, Beom Sik

PY - 2008/3/29

Y1 - 2008/3/29

N2 - Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS-DevR is a two-component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting-drop vapour-diffusion method in the presence of sodium citrate and 2-propanol as precipitants. X-ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 Å on a synchrotron beamline. The crystals belong to the hexagonal space group P61. The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 Å3 Da-1. The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress.

AB - Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS-DevR is a two-component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting-drop vapour-diffusion method in the presence of sodium citrate and 2-propanol as precipitants. X-ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 Å on a synchrotron beamline. The crystals belong to the hexagonal space group P61. The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 Å3 Da-1. The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress.

KW - GAF domain

KW - Hypoxia sensing

KW - Mycobacteria

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DO - 10.1107/S1744309108005186

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JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

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IS - 4

ER -

Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis

Abstract

Keywords

UN SDGs

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