CXC chemokine ligand 2 induced by receptor activator of NF-κB ligand enhances osteoclastogenesis

Jeongim Ha, Hyo Sun Choi, Youngkyun Lee, Hyung Joo Kwon, Yeong Wook Song, Hong Hee Kim

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

CXCL2 has been known to regulate immune functions mainly by chemo-attracting neutrophils. In this study, we show that CXCL2 can be induced by receptor activator of NF-κB ligand, the osteoclast (OC) differentiation factor, through JNK and NF-κB signaling pathways in OC precursor cells. CXCL2 in turn enhanced the proliferation of OC precursor cells of bone marrow-derived macrophages (BMMs) through the activation of ERK. Knockdown of CXCL2 inhibited both the proliferation of and the ERK activation in BMMs. During osteoclastogenesis CXCL2 stimulated the adhesion and the migration of BMMs. Moreover, the formation of OCs from BMMs was significantly increased on treatment with CXCL2. Conversely, the CXCL2 antagonist repertaxin and a CXCL2 neutralizing Ab potently reduced receptor activator of NF-κB ligand-induced osteoclastogenesis. Furthermore, CXCL2 evoked fulminant bone erosion in the in vivo mouse experiments. Finally, prominent upregulation of CXCL2 was detected in synovial fluids and sera from rheumatoid arthritis patients, suggesting a potential involvement of CXCL2-mediated osteoclastogenesis in rheumatoid arthritis-associated bone destruction. Thus, CXCL2 is a novel therapeutic target for inflammatory bone destructive diseases.

Original languageEnglish
Pages (from-to)4717-4724
Number of pages8
JournalJournal of Immunology
Volume184
Issue number9
DOIs
StatePublished - 1 May 2010

Fingerprint

Dive into the research topics of 'CXC chemokine ligand 2 induced by receptor activator of NF-κB ligand enhances osteoclastogenesis'. Together they form a unique fingerprint.

Cite this