Cyclosporin A Enhances Cardiac Differentiation by Inhibiting Wnt/β-Catenin Signaling in Human Embryonic Stem Cells

Mu Seog Choe; Han Cheol Yeo; Jae Boum Youm; Seung Hak Choi; Won Young Choi; So Jin Kim; Seung Tack Oh; Ho Jae Han; Kyung Min Baek; Joong Sun Kim; Kyung Seob Lim; Woochul Chang; Min Young Lee

doi:10.1007/s12257-020-0296-y

Cyclosporin A Enhances Cardiac Differentiation by Inhibiting Wnt/β-Catenin Signaling in Human Embryonic Stem Cells

Mu Seog Choe, Han Cheol Yeo, Jae Boum Youm, Seung Hak Choi, Won Young Choi, So Jin Kim, Seung Tack Oh, Ho Jae Han, Kyung Min Baek, Joong Sun Kim, Kyung Seob Lim, Woochul Chang, Min Young Lee

Research output: Contribution to journal › Article › peer-review

Abstract

Efficient cardiac differentiation of human pluripotent stem cells (hPSCs) is essential for their use in the field of basic research as well as in cell-based therapy. Regulation of the Wnt/β-catenin pathway is a key step in the process of cardiac differentiation of hPSCs. In this study, we reported cyclosporin A (CsA), a strong immunosuppressor, as an inducer of cardiac differentiation in human embryonic stem cells (hESCs). Our results showed that CsA promoted cardiac differentiation in the mesodermal differentiation stage of hESCs and that this effect was mediated by the inhibition of Wnt/β-catenin signaling. Therefore, our results provide novel information about CsA as a Wnt/β-catenin inhibitor as well as a strategy for the cardiac differentiation of hESCs.

Original language	English
Pages (from-to)	786-794
Number of pages	9
Journal	Biotechnology and Bioprocess Engineering
Volume	26
Issue number	5
DOIs	https://doi.org/10.1007/s12257-020-0296-y
State	Published - Oct 2021

Keywords

cardiac differentiation
cyclosporin A
human embryonic stem cells
Wnt/β-catenin signaling

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Choe, M. S., Yeo, H. C., Youm, J. B., Choi, S. H., Choi, W. Y., Kim, S. J., Oh, S. T., Han, H. J., Baek, K. M., Kim, J. S., Lim, K. S., Chang, W., & Lee, M. Y. (2021). Cyclosporin A Enhances Cardiac Differentiation by Inhibiting Wnt/β-Catenin Signaling in Human Embryonic Stem Cells. Biotechnology and Bioprocess Engineering, 26(5), 786-794. https://doi.org/10.1007/s12257-020-0296-y

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title = "Cyclosporin A Enhances Cardiac Differentiation by Inhibiting Wnt/β-Catenin Signaling in Human Embryonic Stem Cells",

abstract = "Efficient cardiac differentiation of human pluripotent stem cells (hPSCs) is essential for their use in the field of basic research as well as in cell-based therapy. Regulation of the Wnt/β-catenin pathway is a key step in the process of cardiac differentiation of hPSCs. In this study, we reported cyclosporin A (CsA), a strong immunosuppressor, as an inducer of cardiac differentiation in human embryonic stem cells (hESCs). Our results showed that CsA promoted cardiac differentiation in the mesodermal differentiation stage of hESCs and that this effect was mediated by the inhibition of Wnt/β-catenin signaling. Therefore, our results provide novel information about CsA as a Wnt/β-catenin inhibitor as well as a strategy for the cardiac differentiation of hESCs.",

keywords = "cardiac differentiation, cyclosporin A, human embryonic stem cells, Wnt/β-catenin signaling",

author = "Choe, {Mu Seog} and Yeo, {Han Cheol} and Youm, {Jae Boum} and Choi, {Seung Hak} and Choi, {Won Young} and Kim, {So Jin} and Oh, {Seung Tack} and Han, {Ho Jae} and Baek, {Kyung Min} and Kim, {Joong Sun} and Lim, {Kyung Seob} and Woochul Chang and Lee, {Min Young}",

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year = "2021",

month = oct,

doi = "10.1007/s12257-020-0296-y",

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T1 - Cyclosporin A Enhances Cardiac Differentiation by Inhibiting Wnt/β-Catenin Signaling in Human Embryonic Stem Cells

AU - Choe, Mu Seog

AU - Yeo, Han Cheol

AU - Youm, Jae Boum

AU - Choi, Seung Hak

AU - Choi, Won Young

AU - Kim, So Jin

AU - Oh, Seung Tack

AU - Han, Ho Jae

AU - Baek, Kyung Min

AU - Kim, Joong Sun

AU - Lim, Kyung Seob

AU - Chang, Woochul

AU - Lee, Min Young

PY - 2021/10

Y1 - 2021/10

N2 - Efficient cardiac differentiation of human pluripotent stem cells (hPSCs) is essential for their use in the field of basic research as well as in cell-based therapy. Regulation of the Wnt/β-catenin pathway is a key step in the process of cardiac differentiation of hPSCs. In this study, we reported cyclosporin A (CsA), a strong immunosuppressor, as an inducer of cardiac differentiation in human embryonic stem cells (hESCs). Our results showed that CsA promoted cardiac differentiation in the mesodermal differentiation stage of hESCs and that this effect was mediated by the inhibition of Wnt/β-catenin signaling. Therefore, our results provide novel information about CsA as a Wnt/β-catenin inhibitor as well as a strategy for the cardiac differentiation of hESCs.

AB - Efficient cardiac differentiation of human pluripotent stem cells (hPSCs) is essential for their use in the field of basic research as well as in cell-based therapy. Regulation of the Wnt/β-catenin pathway is a key step in the process of cardiac differentiation of hPSCs. In this study, we reported cyclosporin A (CsA), a strong immunosuppressor, as an inducer of cardiac differentiation in human embryonic stem cells (hESCs). Our results showed that CsA promoted cardiac differentiation in the mesodermal differentiation stage of hESCs and that this effect was mediated by the inhibition of Wnt/β-catenin signaling. Therefore, our results provide novel information about CsA as a Wnt/β-catenin inhibitor as well as a strategy for the cardiac differentiation of hESCs.

KW - cardiac differentiation

KW - cyclosporin A

KW - human embryonic stem cells

KW - Wnt/β-catenin signaling

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SN - 1226-8372

VL - 26

SP - 786

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JO - Biotechnology and Bioprocess Engineering

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ER -

Cyclosporin A Enhances Cardiac Differentiation by Inhibiting Wnt/β-Catenin Signaling in Human Embryonic Stem Cells

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