TY - JOUR
T1 - Cytotoxic and anticancer properties of new ruthenium polypyridyl complexes with different lipophilicities
AU - Florence Tikum, Anjong
AU - Jeon, Yu Jeong
AU - Lee, Ju Hyun
AU - Park, Min Hee
AU - Bae, In Yeong
AU - Kim, Sang Heon
AU - Lee, Hye Jin
AU - Kim, Jinheung
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/3
Y1 - 2018/3
N2 - Three ruthenium complexes containing a bidentate piq ligand, [(piq)Ru(bpy)2]2+ (1), [(piq)Ru(phen)2]2+ (2), and [(piq)Ru(DIP)2]2+ (3) (piq = phenylisoquinolinate, bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, DIP = 4,7-diphenyl-1,10-phenanthroline), were prepared. The DNA binding properties of complexes 1–3 to double-stranded DNA were studied. The binding of 1–3 to calf-thymus DNA (ct-DNA) yielded lower emission intensities than those observed with the corresponding Ru complexes alone. To explore potential interactions of complexes 1–3 with lipid-rich organs in live cells, the emission properties of the Ru probes were studied with liposomes. The emission intensities of complexes 1–3 were enhanced to similar extents upon interaction with liposomes. The cytotoxic activities of the complexes against MDA-MB-231 and HUVECs were evaluated in vitro. The effects of complexes 1–3 on the survival of MDA-MB-231 cells were examined and compared with that of cis-platin. Complexes 2 and 3 were more cytotoxic to cancer cells than cis-platin. Complexes 1–3 showed cellular uptakes of 1.1, 10.6, and 76.6%, respectively, indicating that the greatest amount of complex 3 entered the cancer cells. Inhibition of cell migration by complexes 1–3 was also evaluated by the wound healing assay.
AB - Three ruthenium complexes containing a bidentate piq ligand, [(piq)Ru(bpy)2]2+ (1), [(piq)Ru(phen)2]2+ (2), and [(piq)Ru(DIP)2]2+ (3) (piq = phenylisoquinolinate, bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, DIP = 4,7-diphenyl-1,10-phenanthroline), were prepared. The DNA binding properties of complexes 1–3 to double-stranded DNA were studied. The binding of 1–3 to calf-thymus DNA (ct-DNA) yielded lower emission intensities than those observed with the corresponding Ru complexes alone. To explore potential interactions of complexes 1–3 with lipid-rich organs in live cells, the emission properties of the Ru probes were studied with liposomes. The emission intensities of complexes 1–3 were enhanced to similar extents upon interaction with liposomes. The cytotoxic activities of the complexes against MDA-MB-231 and HUVECs were evaluated in vitro. The effects of complexes 1–3 on the survival of MDA-MB-231 cells were examined and compared with that of cis-platin. Complexes 2 and 3 were more cytotoxic to cancer cells than cis-platin. Complexes 1–3 showed cellular uptakes of 1.1, 10.6, and 76.6%, respectively, indicating that the greatest amount of complex 3 entered the cancer cells. Inhibition of cell migration by complexes 1–3 was also evaluated by the wound healing assay.
KW - Anticancer activity
KW - Cell migration
KW - Cellular uptake
KW - Cytotoxic activity
KW - Polypyridyl ruthenium complex
UR - http://www.scopus.com/inward/record.url?scp=85044371621&partnerID=8YFLogxK
U2 - 10.1016/j.jinorgbio.2018.01.003
DO - 10.1016/j.jinorgbio.2018.01.003
M3 - Article
C2 - 29329027
AN - SCOPUS:85044371621
SN - 0162-0134
VL - 180
SP - 204
EP - 210
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
ER -
