Cytotoxic and anticancer properties of new ruthenium polypyridyl complexes with different lipophilicities

Anjong Florence Tikum; Yu Jeong Jeon; Ju Hyun Lee; Min Hee Park; In Yeong Bae; Sang Heon Kim; Hye Jin Lee; Jinheung Kim

doi:10.1016/j.jinorgbio.2018.01.003

Cytotoxic and anticancer properties of new ruthenium polypyridyl complexes with different lipophilicities

Anjong Florence Tikum
, Yu Jeong Jeon
, Ju Hyun Lee
, Min Hee Park
, In Yeong Bae
, Sang Heon Kim
, Hye Jin Lee
, Jinheung Kim

Ewha Womans University
Korea Institute of Science and Technology
University of Science and Technology UST

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Three ruthenium complexes containing a bidentate piq ligand, [(piq)Ru(bpy)₂]²⁺ (1), [(piq)Ru(phen)₂]²⁺ (2), and [(piq)Ru(DIP)₂]²⁺ (3) (piq = phenylisoquinolinate, bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, DIP = 4,7-diphenyl-1,10-phenanthroline), were prepared. The DNA binding properties of complexes 1–3 to double-stranded DNA were studied. The binding of 1–3 to calf-thymus DNA (ct-DNA) yielded lower emission intensities than those observed with the corresponding Ru complexes alone. To explore potential interactions of complexes 1–3 with lipid-rich organs in live cells, the emission properties of the Ru probes were studied with liposomes. The emission intensities of complexes 1–3 were enhanced to similar extents upon interaction with liposomes. The cytotoxic activities of the complexes against MDA-MB-231 and HUVECs were evaluated in vitro. The effects of complexes 1–3 on the survival of MDA-MB-231 cells were examined and compared with that of cis-platin. Complexes 2 and 3 were more cytotoxic to cancer cells than cis-platin. Complexes 1–3 showed cellular uptakes of 1.1, 10.6, and 76.6%, respectively, indicating that the greatest amount of complex 3 entered the cancer cells. Inhibition of cell migration by complexes 1–3 was also evaluated by the wound healing assay.

Original language	English
Pages (from-to)	204-210
Number of pages	7
Journal	Journal of Inorganic Biochemistry
Volume	180
DOIs	https://doi.org/10.1016/j.jinorgbio.2018.01.003
State	Published - Mar 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Anticancer activity
Cell migration
Cellular uptake
Cytotoxic activity
Polypyridyl ruthenium complex

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10.1016/j.jinorgbio.2018.01.003

Cite this

@article{2c5537ad2e5f46c483e9ce8197af5740,

title = "Cytotoxic and anticancer properties of new ruthenium polypyridyl complexes with different lipophilicities",

abstract = "Three ruthenium complexes containing a bidentate piq ligand, [(piq)Ru(bpy)2]2+ (1), [(piq)Ru(phen)2]2+ (2), and [(piq)Ru(DIP)2]2+ (3) (piq = phenylisoquinolinate, bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, DIP = 4,7-diphenyl-1,10-phenanthroline), were prepared. The DNA binding properties of complexes 1–3 to double-stranded DNA were studied. The binding of 1–3 to calf-thymus DNA (ct-DNA) yielded lower emission intensities than those observed with the corresponding Ru complexes alone. To explore potential interactions of complexes 1–3 with lipid-rich organs in live cells, the emission properties of the Ru probes were studied with liposomes. The emission intensities of complexes 1–3 were enhanced to similar extents upon interaction with liposomes. The cytotoxic activities of the complexes against MDA-MB-231 and HUVECs were evaluated in vitro. The effects of complexes 1–3 on the survival of MDA-MB-231 cells were examined and compared with that of cis-platin. Complexes 2 and 3 were more cytotoxic to cancer cells than cis-platin. Complexes 1–3 showed cellular uptakes of 1.1, 10.6, and 76.6\%, respectively, indicating that the greatest amount of complex 3 entered the cancer cells. Inhibition of cell migration by complexes 1–3 was also evaluated by the wound healing assay.",

keywords = "Anticancer activity, Cell migration, Cellular uptake, Cytotoxic activity, Polypyridyl ruthenium complex",

author = "\{Florence Tikum\}, Anjong and Jeon, \{Yu Jeong\} and Lee, \{Ju Hyun\} and Park, \{Min Hee\} and Bae, \{In Yeong\} and Kim, \{Sang Heon\} and Lee, \{Hye Jin\} and Jinheung Kim",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

year = "2018",

month = mar,

doi = "10.1016/j.jinorgbio.2018.01.003",

language = "English",

volume = "180",

pages = "204--210",

journal = "Journal of Inorganic Biochemistry",

issn = "0162-0134",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Cytotoxic and anticancer properties of new ruthenium polypyridyl complexes with different lipophilicities

AU - Florence Tikum, Anjong

AU - Jeon, Yu Jeong

AU - Lee, Ju Hyun

AU - Park, Min Hee

AU - Bae, In Yeong

AU - Kim, Sang Heon

AU - Lee, Hye Jin

AU - Kim, Jinheung

PY - 2018/3

Y1 - 2018/3

N2 - Three ruthenium complexes containing a bidentate piq ligand, [(piq)Ru(bpy)2]2+ (1), [(piq)Ru(phen)2]2+ (2), and [(piq)Ru(DIP)2]2+ (3) (piq = phenylisoquinolinate, bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, DIP = 4,7-diphenyl-1,10-phenanthroline), were prepared. The DNA binding properties of complexes 1–3 to double-stranded DNA were studied. The binding of 1–3 to calf-thymus DNA (ct-DNA) yielded lower emission intensities than those observed with the corresponding Ru complexes alone. To explore potential interactions of complexes 1–3 with lipid-rich organs in live cells, the emission properties of the Ru probes were studied with liposomes. The emission intensities of complexes 1–3 were enhanced to similar extents upon interaction with liposomes. The cytotoxic activities of the complexes against MDA-MB-231 and HUVECs were evaluated in vitro. The effects of complexes 1–3 on the survival of MDA-MB-231 cells were examined and compared with that of cis-platin. Complexes 2 and 3 were more cytotoxic to cancer cells than cis-platin. Complexes 1–3 showed cellular uptakes of 1.1, 10.6, and 76.6%, respectively, indicating that the greatest amount of complex 3 entered the cancer cells. Inhibition of cell migration by complexes 1–3 was also evaluated by the wound healing assay.

AB - Three ruthenium complexes containing a bidentate piq ligand, [(piq)Ru(bpy)2]2+ (1), [(piq)Ru(phen)2]2+ (2), and [(piq)Ru(DIP)2]2+ (3) (piq = phenylisoquinolinate, bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, DIP = 4,7-diphenyl-1,10-phenanthroline), were prepared. The DNA binding properties of complexes 1–3 to double-stranded DNA were studied. The binding of 1–3 to calf-thymus DNA (ct-DNA) yielded lower emission intensities than those observed with the corresponding Ru complexes alone. To explore potential interactions of complexes 1–3 with lipid-rich organs in live cells, the emission properties of the Ru probes were studied with liposomes. The emission intensities of complexes 1–3 were enhanced to similar extents upon interaction with liposomes. The cytotoxic activities of the complexes against MDA-MB-231 and HUVECs were evaluated in vitro. The effects of complexes 1–3 on the survival of MDA-MB-231 cells were examined and compared with that of cis-platin. Complexes 2 and 3 were more cytotoxic to cancer cells than cis-platin. Complexes 1–3 showed cellular uptakes of 1.1, 10.6, and 76.6%, respectively, indicating that the greatest amount of complex 3 entered the cancer cells. Inhibition of cell migration by complexes 1–3 was also evaluated by the wound healing assay.

KW - Anticancer activity

KW - Cell migration

KW - Cellular uptake

KW - Cytotoxic activity

KW - Polypyridyl ruthenium complex

UR - https://www.scopus.com/pages/publications/85044371621

U2 - 10.1016/j.jinorgbio.2018.01.003

DO - 10.1016/j.jinorgbio.2018.01.003

M3 - Article

C2 - 29329027

AN - SCOPUS:85044371621

SN - 0162-0134

VL - 180

SP - 204

EP - 210

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

ER -

Cytotoxic and anticancer properties of new ruthenium polypyridyl complexes with different lipophilicities

Abstract

UN SDGs

Keywords

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