DC electric-field-induced DNA stretching for AFM and SNOM studies

J. M. Kim; T. Ohtani; J. Y. Park; S. M. Chang; H. Muramatsu

doi:10.1016/S0304-3991(02)00093-1

DC electric-field-induced DNA stretching for AFM and SNOM studies

J. M. Kim
, T. Ohtani
, J. Y. Park
, S. M. Chang
, H. Muramatsu

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

An effective method of DNA stretching on mica surfaces is proposed for an extremely low concentration of DNA. The method is based on an electric field and well applied on the concentration range from 57×10^-3 to 57×10^-6ng/ml. The stretching exists in a gap between positive and negative electrodes. The difference in the stretching efficiency among the different surfaces of bare mica, Mg²⁺ soaked mica and AP-mica is discussed. The best performance of the stretching is found from the surface of AP-mica for the same experimental condition of sample concentration and applied voltage. Finally, from a Scanning near-field optical microscope image, it is found that well-stretched DNA molecules have shown more similar optical resolution, which is inferred from an optical fiber probe, itself.

Original language	English
Pages (from-to)	139-149
Number of pages	11
Journal	Ultramicroscopy
Volume	91
Issue number	1-4
DOIs	https://doi.org/10.1016/S0304-3991(02)00093-1
State	Published - 2002

Keywords

AFM
DNA
NSOM
SNOM

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10.1016/S0304-3991(02)00093-1

Cite this

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title = "DC electric-field-induced DNA stretching for AFM and SNOM studies",

abstract = "An effective method of DNA stretching on mica surfaces is proposed for an extremely low concentration of DNA. The method is based on an electric field and well applied on the concentration range from 57×10-3 to 57×10-6ng/ml. The stretching exists in a gap between positive and negative electrodes. The difference in the stretching efficiency among the different surfaces of bare mica, Mg2+ soaked mica and AP-mica is discussed. The best performance of the stretching is found from the surface of AP-mica for the same experimental condition of sample concentration and applied voltage. Finally, from a Scanning near-field optical microscope image, it is found that well-stretched DNA molecules have shown more similar optical resolution, which is inferred from an optical fiber probe, itself.",

keywords = "AFM, DNA, NSOM, SNOM",

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T1 - DC electric-field-induced DNA stretching for AFM and SNOM studies

AU - Kim, J. M.

AU - Ohtani, T.

AU - Park, J. Y.

AU - Chang, S. M.

AU - Muramatsu, H.

PY - 2002

Y1 - 2002

N2 - An effective method of DNA stretching on mica surfaces is proposed for an extremely low concentration of DNA. The method is based on an electric field and well applied on the concentration range from 57×10-3 to 57×10-6ng/ml. The stretching exists in a gap between positive and negative electrodes. The difference in the stretching efficiency among the different surfaces of bare mica, Mg2+ soaked mica and AP-mica is discussed. The best performance of the stretching is found from the surface of AP-mica for the same experimental condition of sample concentration and applied voltage. Finally, from a Scanning near-field optical microscope image, it is found that well-stretched DNA molecules have shown more similar optical resolution, which is inferred from an optical fiber probe, itself.

AB - An effective method of DNA stretching on mica surfaces is proposed for an extremely low concentration of DNA. The method is based on an electric field and well applied on the concentration range from 57×10-3 to 57×10-6ng/ml. The stretching exists in a gap between positive and negative electrodes. The difference in the stretching efficiency among the different surfaces of bare mica, Mg2+ soaked mica and AP-mica is discussed. The best performance of the stretching is found from the surface of AP-mica for the same experimental condition of sample concentration and applied voltage. Finally, from a Scanning near-field optical microscope image, it is found that well-stretched DNA molecules have shown more similar optical resolution, which is inferred from an optical fiber probe, itself.

KW - AFM

KW - DNA

KW - NSOM

KW - SNOM

UR - https://www.scopus.com/pages/publications/0036326623

U2 - 10.1016/S0304-3991(02)00093-1

DO - 10.1016/S0304-3991(02)00093-1

M3 - Article

C2 - 12211462

AN - SCOPUS:0036326623

SN - 0304-3991

VL - 91

SP - 139

EP - 149

JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 1-4

ER -

DC electric-field-induced DNA stretching for AFM and SNOM studies

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Keywords

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