Deinococcus knuensis sp. nov., a bacterium isolated from river water

Jae Jin Lee; Yeon Hee Lee; Su Jin Park; Seung Yeol Lee; Sangkyu Park; Dae Sung Lee; In Kyu Kang; Leonid N. Ten; Hee Young Jung

doi:10.1007/s10482-016-0813-3

Deinococcus knuensis sp. nov., a bacterium isolated from river water

Jae Jin Lee, Yeon Hee Lee, Su Jin Park, Seung Yeol Lee, Sangkyu Park, Dae Sung Lee, In Kyu Kang, Leonid N. Ten, Hee Young Jung

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Strain 16F3H^T, a Gram-negative, aerobic, non-motile, and oval-shaped bacterium, was isolated from river water collected from the Han River in South Korea. Growth of strain 16F3H^T was observed at 10–42 °C (optimum at 25–30 °C), but no growth occurred at 4 °C. The strain is able to grow at pH 4–10 (optimum at pH 7–8) and tolerates up to 4% NaCl (w/v), with optimum growth at 0.5% NaCl. The isolate was found to be resistant to UV irradiation. Based on 16S rRNA gene sequence analysis, it is closely related to ‘Deinococcus seoulensis’ 16F1E (98.8%), Deinococcus aquaticus PB314^T (98.1%) and Deinococcus caeni Ho-08^T (98.0%). The level of DNA–DNA homology between the novel strain and the three related strains was 57.4, 41.2, and 35.8%, respectively. Chemotaxonomic data revealed that strain 16F3H^T possesses MK-8 as the predominant respiratory quinone, an unidentified phosphoglycolipid as the major polar lipid, and C_15:1ω6c and C_16:1ω7c as the major fatty acids. The DNA G + C content was determined to be 65.7 mol%. Based on polyphasic evidence, strain 16F3H^T (=KCTC 33794^T = JCM 31406^T) should be classified as the type strain of a novel Deinococcus species, for which the name Deinococcus knuensis sp. nov. is proposed.

Original language	English
Pages (from-to)	407-414
Number of pages	8
Journal	Antonie van Leeuwenhoek
Volume	110
Issue number	3
DOIs	https://doi.org/10.1007/s10482-016-0813-3
State	Published - 1 Mar 2017

Keywords

16S rRNA gene
Deinococcus
Polyphasic taxonomy
River water
UV resistance

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10.1007/s10482-016-0813-3

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title = "Deinococcus knuensis sp. nov., a bacterium isolated from river water",

abstract = "Strain 16F3HT, a Gram-negative, aerobic, non-motile, and oval-shaped bacterium, was isolated from river water collected from the Han River in South Korea. Growth of strain 16F3HT was observed at 10–42 °C (optimum at 25–30 °C), but no growth occurred at 4 °C. The strain is able to grow at pH 4–10 (optimum at pH 7–8) and tolerates up to 4% NaCl (w/v), with optimum growth at 0.5% NaCl. The isolate was found to be resistant to UV irradiation. Based on 16S rRNA gene sequence analysis, it is closely related to {\textquoteleft}Deinococcus seoulensis{\textquoteright} 16F1E (98.8%), Deinococcus aquaticus PB314T (98.1%) and Deinococcus caeni Ho-08T (98.0%). The level of DNA–DNA homology between the novel strain and the three related strains was 57.4, 41.2, and 35.8%, respectively. Chemotaxonomic data revealed that strain 16F3HT possesses MK-8 as the predominant respiratory quinone, an unidentified phosphoglycolipid as the major polar lipid, and C15:1ω6c and C16:1ω7c as the major fatty acids. The DNA G + C content was determined to be 65.7 mol%. Based on polyphasic evidence, strain 16F3HT (=KCTC 33794T = JCM 31406T) should be classified as the type strain of a novel Deinococcus species, for which the name Deinococcus knuensis sp. nov. is proposed.",

keywords = "16S rRNA gene, Deinococcus, Polyphasic taxonomy, River water, UV resistance",

author = "Lee, {Jae Jin} and Lee, {Yeon Hee} and Park, {Su Jin} and Lee, {Seung Yeol} and Sangkyu Park and Lee, {Dae Sung} and Kang, {In Kyu} and Ten, {Leonid N.} and Jung, {Hee Young}",

note = "Publisher Copyright: {\textcopyright} 2016, Springer International Publishing Switzerland.",

year = "2017",

month = mar,

day = "1",

doi = "10.1007/s10482-016-0813-3",

language = "English",

volume = "110",

pages = "407--414",

journal = "Antonie van Leeuwenhoek",

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TY - JOUR

T1 - Deinococcus knuensis sp. nov., a bacterium isolated from river water

AU - Lee, Jae Jin

AU - Lee, Yeon Hee

AU - Park, Su Jin

AU - Lee, Seung Yeol

AU - Park, Sangkyu

AU - Lee, Dae Sung

AU - Kang, In Kyu

AU - Ten, Leonid N.

AU - Jung, Hee Young

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Strain 16F3HT, a Gram-negative, aerobic, non-motile, and oval-shaped bacterium, was isolated from river water collected from the Han River in South Korea. Growth of strain 16F3HT was observed at 10–42 °C (optimum at 25–30 °C), but no growth occurred at 4 °C. The strain is able to grow at pH 4–10 (optimum at pH 7–8) and tolerates up to 4% NaCl (w/v), with optimum growth at 0.5% NaCl. The isolate was found to be resistant to UV irradiation. Based on 16S rRNA gene sequence analysis, it is closely related to ‘Deinococcus seoulensis’ 16F1E (98.8%), Deinococcus aquaticus PB314T (98.1%) and Deinococcus caeni Ho-08T (98.0%). The level of DNA–DNA homology between the novel strain and the three related strains was 57.4, 41.2, and 35.8%, respectively. Chemotaxonomic data revealed that strain 16F3HT possesses MK-8 as the predominant respiratory quinone, an unidentified phosphoglycolipid as the major polar lipid, and C15:1ω6c and C16:1ω7c as the major fatty acids. The DNA G + C content was determined to be 65.7 mol%. Based on polyphasic evidence, strain 16F3HT (=KCTC 33794T = JCM 31406T) should be classified as the type strain of a novel Deinococcus species, for which the name Deinococcus knuensis sp. nov. is proposed.

AB - Strain 16F3HT, a Gram-negative, aerobic, non-motile, and oval-shaped bacterium, was isolated from river water collected from the Han River in South Korea. Growth of strain 16F3HT was observed at 10–42 °C (optimum at 25–30 °C), but no growth occurred at 4 °C. The strain is able to grow at pH 4–10 (optimum at pH 7–8) and tolerates up to 4% NaCl (w/v), with optimum growth at 0.5% NaCl. The isolate was found to be resistant to UV irradiation. Based on 16S rRNA gene sequence analysis, it is closely related to ‘Deinococcus seoulensis’ 16F1E (98.8%), Deinococcus aquaticus PB314T (98.1%) and Deinococcus caeni Ho-08T (98.0%). The level of DNA–DNA homology between the novel strain and the three related strains was 57.4, 41.2, and 35.8%, respectively. Chemotaxonomic data revealed that strain 16F3HT possesses MK-8 as the predominant respiratory quinone, an unidentified phosphoglycolipid as the major polar lipid, and C15:1ω6c and C16:1ω7c as the major fatty acids. The DNA G + C content was determined to be 65.7 mol%. Based on polyphasic evidence, strain 16F3HT (=KCTC 33794T = JCM 31406T) should be classified as the type strain of a novel Deinococcus species, for which the name Deinococcus knuensis sp. nov. is proposed.

KW - 16S rRNA gene

KW - Deinococcus

KW - Polyphasic taxonomy

KW - River water

KW - UV resistance

UR - http://www.scopus.com/inward/record.url?scp=85006765722&partnerID=8YFLogxK

U2 - 10.1007/s10482-016-0813-3

DO - 10.1007/s10482-016-0813-3

M3 - Article

C2 - 28000055

AN - SCOPUS:85006765722

SN - 0003-6072

VL - 110

SP - 407

EP - 414

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

IS - 3

ER -

Deinococcus knuensis sp. nov., a bacterium isolated from river water

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