Deletion of peroxiredoxin II inhibits the growth of mouse primary mesenchymal stem cells through induction of the G0/G1 cell-cycle arrest and activation of AKT/GSK3β/β-catenin signaling

Ying Hao Han, Mei Hua Jin, Ying Hua Jin, Nan Nan Yu, Jun Liu, Yong Qing Zhang, Yu Dong Cui, Ai Guo Wang, Dong Seok Lee, Sun Uk Kim, Ji Su Kim, Taeho Kwon, Hu Nan Sun

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Background/Aim: Dermal mesenchymal stem cells (DMSCs) are pluripotent stem cells found in the skin which maintain the thickness of the dermal layer and participate in skin wound healing. Materials and Methods: The MTT assay was performed to detect cell proliferation and cell-cycle progression and cell-surface markers were assessed by flow cytometry. The levels of proteins in related signaling pathways were detected by western blotting assay and the translocation of β-catenin into the nucleus were detected by immunofluorescence. Red oil O staining was performed to examine the differentiational ability of DMSCs. Results: Knockout of PRDX2 inhibited DMSC cell growth, and cell-cycle arrest at G0/G1 phase; p16, p21 and cyclin D1 expression levels in Prdx2 knockout DMSCs were significantly increased. Furthermore, AKT phosphorylation were significantly increased in Prdx2 knockout DMSCs, GSK3β activity were inhibited, result in β-Catenin accumulated in the nucleus. Conclusion: In conclusion, these results demonstrated that PRDX2 plays a pivotal role in regulating the proliferation of DMSCs, and this is closely related to the AKT/glycogen synthase kinase 3 beta/β-catenin signaling pathway.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalIn Vivo
Volume34
Issue number1
DOIs
StatePublished - 2020

Keywords

  • Cell cycle
  • Glycogen synthase kinase 3 beta/β-catenin signaling
  • Mesenchymal stem cells
  • Peroxiredoxin II

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