Dependence of the base sequence on the [Cu(2,2′-bipyridine)2(NO3)](NO3)-induced oxidative DNA cleavage probed by linear dichroism

A. Reum Won; Raeyeong Kim; Maeng Joon Jung; Seog K. Kim; Young Ae Lee

doi:10.1016/j.ica.2017.10.009

Dependence of the base sequence on the [Cu(2,2′-bipyridine)₂(NO₃)](NO₃)-induced oxidative DNA cleavage probed by linear dichroism

A. Reum Won, Raeyeong Kim, Maeng Joon Jung, Seog K. Kim, Young Ae Lee

Yeungnam University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The oxidative DNA cleavage induced by the [Cu(2,2′-bipyridine)₂(NO₃)]NO₃ (Cu(bpy)₂) complex was examined using the linear dichroism (LD) technique. Using this method, the oxidative DNA cleavage by the Fenton mechanism was reported to occur through two sequential first-order reactions. As the Cu(bpy)₂ concentration increased, the rate constant of both first order reactions increased, as expected. The activation energy of the second step was estimated to be 0.190 kJ mol⁻¹. A similar method was applied for various synthetic polynucleotides. Poly[d(G-C)₂], poly(dG)·poly(dC), and poly(dA)·poly(dT) produced a time-dependent decrease in LD, which could be elucidated by a single component exponential decay. This observation is in contrast to Fenton-type oxidative DNA cleavage. On the other hand, poly[d(A-T)₂] produced a time-dependent decrease in the LD magnitude, which could be elucidated by two sequential first order reactions.

Original language	English
Pages (from-to)	34-39
Number of pages	6
Journal	Inorganica Chimica Acta
Volume	471
DOIs	https://doi.org/10.1016/j.ica.2017.10.009
State	Published - 24 Feb 2018

Keywords

DNA
Linear dichroism
Oxidative DNA cleavage
[Cu(2,2′-bipyridine)(NO)]NO

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10.1016/j.ica.2017.10.009

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@article{b3367ec6b8e947d291e35331819492fc,

title = "Dependence of the base sequence on the [Cu(2,2′-bipyridine)2(NO3)](NO3)-induced oxidative DNA cleavage probed by linear dichroism",

abstract = "The oxidative DNA cleavage induced by the [Cu(2,2′-bipyridine)2(NO3)]NO3 (Cu(bpy)2) complex was examined using the linear dichroism (LD) technique. Using this method, the oxidative DNA cleavage by the Fenton mechanism was reported to occur through two sequential first-order reactions. As the Cu(bpy)2 concentration increased, the rate constant of both first order reactions increased, as expected. The activation energy of the second step was estimated to be 0.190 kJ mol−1. A similar method was applied for various synthetic polynucleotides. Poly[d(G-C)2], poly(dG)·poly(dC), and poly(dA)·poly(dT) produced a time-dependent decrease in LD, which could be elucidated by a single component exponential decay. This observation is in contrast to Fenton-type oxidative DNA cleavage. On the other hand, poly[d(A-T)2] produced a time-dependent decrease in the LD magnitude, which could be elucidated by two sequential first order reactions.",

keywords = "DNA, Linear dichroism, Oxidative DNA cleavage, [Cu(2,2′-bipyridine)(NO)]NO",

author = "Won, {A. Reum} and Raeyeong Kim and Jung, {Maeng Joon} and Kim, {Seog K.} and Lee, {Young Ae}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2018",

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day = "24",

doi = "10.1016/j.ica.2017.10.009",

language = "English",

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T1 - Dependence of the base sequence on the [Cu(2,2′-bipyridine)2(NO3)](NO3)-induced oxidative DNA cleavage probed by linear dichroism

AU - Won, A. Reum

AU - Kim, Raeyeong

AU - Jung, Maeng Joon

AU - Kim, Seog K.

AU - Lee, Young Ae

PY - 2018/2/24

Y1 - 2018/2/24

N2 - The oxidative DNA cleavage induced by the [Cu(2,2′-bipyridine)2(NO3)]NO3 (Cu(bpy)2) complex was examined using the linear dichroism (LD) technique. Using this method, the oxidative DNA cleavage by the Fenton mechanism was reported to occur through two sequential first-order reactions. As the Cu(bpy)2 concentration increased, the rate constant of both first order reactions increased, as expected. The activation energy of the second step was estimated to be 0.190 kJ mol−1. A similar method was applied for various synthetic polynucleotides. Poly[d(G-C)2], poly(dG)·poly(dC), and poly(dA)·poly(dT) produced a time-dependent decrease in LD, which could be elucidated by a single component exponential decay. This observation is in contrast to Fenton-type oxidative DNA cleavage. On the other hand, poly[d(A-T)2] produced a time-dependent decrease in the LD magnitude, which could be elucidated by two sequential first order reactions.

AB - The oxidative DNA cleavage induced by the [Cu(2,2′-bipyridine)2(NO3)]NO3 (Cu(bpy)2) complex was examined using the linear dichroism (LD) technique. Using this method, the oxidative DNA cleavage by the Fenton mechanism was reported to occur through two sequential first-order reactions. As the Cu(bpy)2 concentration increased, the rate constant of both first order reactions increased, as expected. The activation energy of the second step was estimated to be 0.190 kJ mol−1. A similar method was applied for various synthetic polynucleotides. Poly[d(G-C)2], poly(dG)·poly(dC), and poly(dA)·poly(dT) produced a time-dependent decrease in LD, which could be elucidated by a single component exponential decay. This observation is in contrast to Fenton-type oxidative DNA cleavage. On the other hand, poly[d(A-T)2] produced a time-dependent decrease in the LD magnitude, which could be elucidated by two sequential first order reactions.

KW - DNA

KW - Linear dichroism

KW - Oxidative DNA cleavage

KW - [Cu(2,2′-bipyridine)(NO)]NO

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DO - 10.1016/j.ica.2017.10.009

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AN - SCOPUS:85032503312

SN - 0020-1693

VL - 471

SP - 34

EP - 39

JO - Inorganica Chimica Acta

JF - Inorganica Chimica Acta

ER -

Dependence of the base sequence on the [Cu(2,2′-bipyridine)₂(NO₃)](NO₃)-induced oxidative DNA cleavage probed by linear dichroism

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