Detection of Babesia gibsoni by reaction of phage display single chain antibodies with P50 proteins

Yoshino Hirose; Saeka Matsuhira; Tatiana Alexandrovna Batanova; Youn Kyoung Goo; Mohamad A. Terkawi; Yoshifumi Nishikawa; Katsuya Kitoh; Xuenan Xuan; Yasuhiro Takashima

doi:10.1292/jvms.71.1133

Detection of Babesia gibsoni by reaction of phage display single chain antibodies with P50 proteins

Yoshino Hirose, Saeka Matsuhira, Tatiana Alexandrovna Batanova, Youn Kyoung Goo, Mohamad A. Terkawi, Yoshifumi Nishikawa, Katsuya Kitoh, Xuenan Xuan, Yasuhiro Takashima

Department of Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Babesia gibsoni (B. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. Diagnosis of canine babesiosis is commonly carried out using Giemsa-stained thin blood smears. However, at low levels of infection, it is difficult to detect Babesia organisms by observation of Giemsa-stained thin blood smears. We constructed a monoclonal phage display single chain antibody (scFv) against a B. gibsoni merozoite antigen, P50 protein. Intraerythrocytic B. gibsoni organisms are clearly stained using this antibody. The monoclonal scFv facilitated the detection of B. gibsoni organisms in canine blood samples.

Original language	English
Pages (from-to)	1133-1136
Number of pages	4
Journal	Journal of Veterinary Medical Science
Volume	71
Issue number	8
DOIs	https://doi.org/10.1292/jvms.71.1133
State	Published - Aug 2009

Keywords

Antibodies
Babesia
Phage display

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10.1292/jvms.71.1133

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title = "Detection of Babesia gibsoni by reaction of phage display single chain antibodies with P50 proteins",

abstract = "Babesia gibsoni (B. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. Diagnosis of canine babesiosis is commonly carried out using Giemsa-stained thin blood smears. However, at low levels of infection, it is difficult to detect Babesia organisms by observation of Giemsa-stained thin blood smears. We constructed a monoclonal phage display single chain antibody (scFv) against a B. gibsoni merozoite antigen, P50 protein. Intraerythrocytic B. gibsoni organisms are clearly stained using this antibody. The monoclonal scFv facilitated the detection of B. gibsoni organisms in canine blood samples.",

keywords = "Antibodies, Babesia, Phage display",

author = "Yoshino Hirose and Saeka Matsuhira and Batanova, {Tatiana Alexandrovna} and Goo, {Youn Kyoung} and Terkawi, {Mohamad A.} and Yoshifumi Nishikawa and Katsuya Kitoh and Xuenan Xuan and Yasuhiro Takashima",

year = "2009",

month = aug,

doi = "10.1292/jvms.71.1133",

language = "English",

volume = "71",

pages = "1133--1136",

journal = "Journal of Veterinary Medical Science",

issn = "0916-7250",

publisher = "Japanese Society of Veterinary Science",

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TY - JOUR

T1 - Detection of Babesia gibsoni by reaction of phage display single chain antibodies with P50 proteins

AU - Hirose, Yoshino

AU - Matsuhira, Saeka

AU - Batanova, Tatiana Alexandrovna

AU - Goo, Youn Kyoung

AU - Terkawi, Mohamad A.

AU - Nishikawa, Yoshifumi

AU - Kitoh, Katsuya

AU - Xuan, Xuenan

AU - Takashima, Yasuhiro

PY - 2009/8

Y1 - 2009/8

N2 - Babesia gibsoni (B. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. Diagnosis of canine babesiosis is commonly carried out using Giemsa-stained thin blood smears. However, at low levels of infection, it is difficult to detect Babesia organisms by observation of Giemsa-stained thin blood smears. We constructed a monoclonal phage display single chain antibody (scFv) against a B. gibsoni merozoite antigen, P50 protein. Intraerythrocytic B. gibsoni organisms are clearly stained using this antibody. The monoclonal scFv facilitated the detection of B. gibsoni organisms in canine blood samples.

AB - Babesia gibsoni (B. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. Diagnosis of canine babesiosis is commonly carried out using Giemsa-stained thin blood smears. However, at low levels of infection, it is difficult to detect Babesia organisms by observation of Giemsa-stained thin blood smears. We constructed a monoclonal phage display single chain antibody (scFv) against a B. gibsoni merozoite antigen, P50 protein. Intraerythrocytic B. gibsoni organisms are clearly stained using this antibody. The monoclonal scFv facilitated the detection of B. gibsoni organisms in canine blood samples.

KW - Antibodies

KW - Babesia

KW - Phage display

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U2 - 10.1292/jvms.71.1133

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SN - 0916-7250

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EP - 1136

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

IS - 8

ER -

Detection of Babesia gibsoni by reaction of phage display single chain antibodies with P50 proteins

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Keywords

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