Detection of naturally aerosolized Mycoplasma hyopneumoniae from the air of selected swine farms

Dereje Damte, Sileshi Belew Yohanes, Md Akil Hossain, Seung Jin Lee, Man Hee Rhee, Young Hoan Kim, Seung Chun Park

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We aimed to detect Mycoplasma hyopneumoniae from the air of selected farms through air filtration-based air sampling using polymerase chain reaction (PCR). Air samples were collected at different locations inside and outside of pig farm rooms on polyethersulfone membrane (0.22 lm), and the presence of M. hyopneumoniae DNA was analyzed by PCR. Furthermore, nasal swab and blood samples were collected from 336 pigs in the same air sampled rooms and were analyzed by PCR and IDEXX ELISA, respectively. The suitability of the air sampling method was validated with analysis of an artificially induced aerosolized avirulent M. hyopneumoniae in an enclosed box showing PCR-positive results. M. hyopneumoniae was detected from air sample of pig farm rooms using PCR. Although the probability of an airborne M. hyopneumoniae causing an infection is not yet confirmed, air sampling PCR results could serve as a tool to assess the spread of M. hyopneumoniae by bioaerosols and the infection dynamics in a herd and between herds.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalAerobiologia
Volume30
Issue number2
DOIs
StatePublished - Jun 2014

Keywords

  • Air sample
  • Mycoplasma hyopneumoniae
  • PCR
  • Swine farm

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