Determination of roxithromycin residues in the flounder muscle with electrospray liquid chromatography-mass spectrometry

Jong Hwan Lim; Beom Su Jang; Rae Kyung Lee; Seung Chun Park; Hyo In Yun

doi:10.1016/S0378-4347(00)00334-0

Determination of roxithromycin residues in the flounder muscle with electrospray liquid chromatography-mass spectrometry

Jong Hwan Lim, Beom Su Jang, Rae Kyung Lee, Seung Chun Park, Hyo In Yun

Department of Veterinary Medicine

Chungnam National University

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A highly sensitive and specific method for the determination of roxithromycin in the flounder muscle by LC-MS was developed and validated. A dichloromethane extract of the sample was separated on C₁₈ reversed-phase column with acetonitrile-50 mM ammonium acetate (80:20, v/v) as the mobile phase and analyzed by LC-MS via atmospheric pressure ionization/electrospray ionization interface. The limit of detection and limit of quantitation were 0.01 and 0.1 ng/g, respectively. Mean recoveries from spiked muscles were 81.1% (ranged from 71.0 to 90.3%) for roxithromycin. The method has been successfully applied to determine roxithromycin in the flounder muscle.

Original language	English
Pages (from-to)	219-225
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	746
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(00)00334-0
State	Published - 15 Sep 2000

Keywords

Roxithromycin

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10.1016/S0378-4347(00)00334-0

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title = "Determination of roxithromycin residues in the flounder muscle with electrospray liquid chromatography-mass spectrometry",

abstract = "A highly sensitive and specific method for the determination of roxithromycin in the flounder muscle by LC-MS was developed and validated. A dichloromethane extract of the sample was separated on C18 reversed-phase column with acetonitrile-50 mM ammonium acetate (80:20, v/v) as the mobile phase and analyzed by LC-MS via atmospheric pressure ionization/electrospray ionization interface. The limit of detection and limit of quantitation were 0.01 and 0.1 ng/g, respectively. Mean recoveries from spiked muscles were 81.1% (ranged from 71.0 to 90.3%) for roxithromycin. The method has been successfully applied to determine roxithromycin in the flounder muscle.",

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T1 - Determination of roxithromycin residues in the flounder muscle with electrospray liquid chromatography-mass spectrometry

AU - Lim, Jong Hwan

AU - Jang, Beom Su

AU - Lee, Rae Kyung

AU - Park, Seung Chun

AU - Yun, Hyo In

PY - 2000/9/15

Y1 - 2000/9/15

N2 - A highly sensitive and specific method for the determination of roxithromycin in the flounder muscle by LC-MS was developed and validated. A dichloromethane extract of the sample was separated on C18 reversed-phase column with acetonitrile-50 mM ammonium acetate (80:20, v/v) as the mobile phase and analyzed by LC-MS via atmospheric pressure ionization/electrospray ionization interface. The limit of detection and limit of quantitation were 0.01 and 0.1 ng/g, respectively. Mean recoveries from spiked muscles were 81.1% (ranged from 71.0 to 90.3%) for roxithromycin. The method has been successfully applied to determine roxithromycin in the flounder muscle.

AB - A highly sensitive and specific method for the determination of roxithromycin in the flounder muscle by LC-MS was developed and validated. A dichloromethane extract of the sample was separated on C18 reversed-phase column with acetonitrile-50 mM ammonium acetate (80:20, v/v) as the mobile phase and analyzed by LC-MS via atmospheric pressure ionization/electrospray ionization interface. The limit of detection and limit of quantitation were 0.01 and 0.1 ng/g, respectively. Mean recoveries from spiked muscles were 81.1% (ranged from 71.0 to 90.3%) for roxithromycin. The method has been successfully applied to determine roxithromycin in the flounder muscle.

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AN - SCOPUS:0034666507

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JF - Journal of Chromatography B: Biomedical Sciences and Applications

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Determination of roxithromycin residues in the flounder muscle with electrospray liquid chromatography-mass spectrometry

Abstract

Keywords

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