Abstract
Tomato yellow leaf curl virus (TYLCV) is a disease that is damaging to tomato production worldwide. Resistance to TYLCV has been intensively investigated, and single resistance genes such as Ty-1 have been widely deployed in breeding programs. However, resistance-breaking incidences are frequently reported, and achieving durable resistance against TYLCV in the field is important. In this study, gene-specific markers for Ty-2 and ty-5, and closely-linked markers for Ty-4 were de-veloped and applied to distinguish TYLCV resistance in various tomato genotypes. Quantitative infectivity assays using both natural infection in the field and artificial inoculation utilizing infec-tious TYLCV clones in a growth chamber were optimized and performed to investigate the individ-ual and cumulative levels of resistance. We confirmed that Ty-2 could also be an effective source of resistance for TYLCV control, together with Ty-1. Improvement of resistance as a result of gene-pyramiding was speculated, and breeding lines including both Ty-1 and Ty-2 showed the strongest resistance in both field and artificial infections.
| Original language | English |
|---|---|
| Article number | 9 |
| Pages (from-to) | 1-18 |
| Number of pages | 18 |
| Journal | Plants |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2021 |
Keywords
- Gene-pyramiding
- Marker-assisted selection
- Resistance gene
- TYLCV
- Tomato