Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein

Mingeun Sagong; Choi Kyu Park; Seong Hee Kim; Sung Up Moon; Seong Cheol Cho; Changhee Lee

doi:10.4142/jvs.2010.11.2.169

Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein

Mingeun Sagong, Choi Kyu Park, Seong Hee Kim, Sung Up Moon, Seong Cheol Cho, Changhee Lee

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.

Original language	English
Pages (from-to)	169-171
Number of pages	3
Journal	Journal of Veterinary Science
Volume	11
Issue number	1
DOIs	https://doi.org/10.4142/jvs.2010.11.2.169
State	Published - Mar 2010

Keywords

Diagnostic reagent
Nucleocapsid protein
PRRSV
Stable BHK cell line

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10.4142/jvs.2010.11.2.169

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abstract = "Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.",

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AU - Moon, Sung Up

AU - Cho, Seong Cheol

AU - Lee, Changhee

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AB - Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.

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Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein

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