Development and efficacy of real-time PCR in the diagnosis of vivax malaria using field samples in the Republic of Korea

Jung Yeon Kim; Youn Kyoung Goo; So Young Ji; Hyun Il Shin; Eun Taek Han; Yeonchul Hong; Dong Il Chung; Shin Hyung Cho; Won Ja Lee

doi:10.1371/journal.pone.0105871

Development and efficacy of real-time PCR in the diagnosis of vivax malaria using field samples in the Republic of Korea

Jung Yeon Kim, Youn Kyoung Goo, So Young Ji, Hyun Il Shin, Eun Taek Han, Yeonchul Hong, Dong Il Chung, Shin Hyung Cho, Won Ja Lee

Department of Medicine

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6 Scopus citations

Abstract

The development of sensitive, rapid, and accurate diagnostic methods for vivax malaria is essential for the effective control of malaria in the Republic of Korea, where vivax malaria patients usually show low parasitemia. In this study, a TaqMan-based real-time polymerase chain reaction (PCR) method was established and compared with other PCR-based assays, including nested PCR, loop-mediated isothermal amplification, and multiplex PCR, using samples from febrile patients with suspected vivax malaria. The established real-time PCR had a high sensitivity (99.6%) and specificity (100%). Therefore, this sensitive, specific, rapid, and quantitative real-time PCR method could be used for the routine diagnosis of vivax malaria in the laboratory of the Korea National Institute of Health.

Original language	English
Article number	e105871
Journal	PLoS ONE
Volume	9
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0105871
State	Published - 22 Aug 2014

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abstract = "The development of sensitive, rapid, and accurate diagnostic methods for vivax malaria is essential for the effective control of malaria in the Republic of Korea, where vivax malaria patients usually show low parasitemia. In this study, a TaqMan-based real-time polymerase chain reaction (PCR) method was established and compared with other PCR-based assays, including nested PCR, loop-mediated isothermal amplification, and multiplex PCR, using samples from febrile patients with suspected vivax malaria. The established real-time PCR had a high sensitivity (99.6%) and specificity (100%). Therefore, this sensitive, specific, rapid, and quantitative real-time PCR method could be used for the routine diagnosis of vivax malaria in the laboratory of the Korea National Institute of Health.",

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AU - Kim, Jung Yeon

AU - Goo, Youn Kyoung

AU - Ji, So Young

AU - Shin, Hyun Il

AU - Han, Eun Taek

AU - Hong, Yeonchul

AU - Chung, Dong Il

AU - Cho, Shin Hyung

AU - Lee, Won Ja

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Development and efficacy of real-time PCR in the diagnosis of vivax malaria using field samples in the Republic of Korea

Abstract

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