Development of an immunoaffinity chromatography and HPLC-UV method for determination of 16 sulfonamides in feed

Ho Jin Kim; Min Hee Jeong; Hye Jin Park; Won Chan Kim; Jang Eok Kim

doi:10.1016/j.foodchem.2015.10.014

Development of an immunoaffinity chromatography and HPLC-UV method for determination of 16 sulfonamides in feed

Ho Jin Kim, Min Hee Jeong, Hye Jin Park, Won Chan Kim, Jang Eok Kim

School of Applied Bioscience

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A novel and simple method for detecting 16 sulfonamides (SAs) in animal feed using high performance liquid chromatography equipped with a photo-diode array detector (HPLC/PDA) and immunoaffinity chromatography was developed. The chromatographic peaks of the 16 SAs were successfully identified by comparing their retention times and UV spectra with reference standards. Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study sulfonamides ranged from 14.1 to 45.0 μg/kg, and the limits of quantification (LOQs) ranged from 46.9 to 150.0 μg/kg. Average recoveries of the 16 SAs ranged from 78.2% to 105.2%. Method replication resulted in intraday and interday peak area variation of <5.5%. The developed method was specific and reliable and is suited for the routine analysis of SAs in animal feed.

Original language	English
Pages (from-to)	1144-1149
Number of pages	6
Journal	Food Chemistry
Volume	196
DOIs	https://doi.org/10.1016/j.foodchem.2015.10.014
State	Published - 1 Apr 2016

Keywords

Animal feed
Immunoaffinity chromatography
Sulfonamide

Access to Document

10.1016/j.foodchem.2015.10.014

Cite this

@article{0675a0a35d3946eb88057a848b3b4ceb,

title = "Development of an immunoaffinity chromatography and HPLC-UV method for determination of 16 sulfonamides in feed",

abstract = "A novel and simple method for detecting 16 sulfonamides (SAs) in animal feed using high performance liquid chromatography equipped with a photo-diode array detector (HPLC/PDA) and immunoaffinity chromatography was developed. The chromatographic peaks of the 16 SAs were successfully identified by comparing their retention times and UV spectra with reference standards. Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study sulfonamides ranged from 14.1 to 45.0 μg/kg, and the limits of quantification (LOQs) ranged from 46.9 to 150.0 μg/kg. Average recoveries of the 16 SAs ranged from 78.2% to 105.2%. Method replication resulted in intraday and interday peak area variation of <5.5%. The developed method was specific and reliable and is suited for the routine analysis of SAs in animal feed.",

keywords = "Animal feed, Immunoaffinity chromatography, Sulfonamide",

author = "Kim, {Ho Jin} and Jeong, {Min Hee} and Park, {Hye Jin} and Kim, {Won Chan} and Kim, {Jang Eok}",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd",

year = "2016",

month = apr,

day = "1",

doi = "10.1016/j.foodchem.2015.10.014",

language = "English",

volume = "196",

pages = "1144--1149",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Development of an immunoaffinity chromatography and HPLC-UV method for determination of 16 sulfonamides in feed

AU - Kim, Ho Jin

AU - Jeong, Min Hee

AU - Park, Hye Jin

AU - Kim, Won Chan

AU - Kim, Jang Eok

PY - 2016/4/1

Y1 - 2016/4/1

N2 - A novel and simple method for detecting 16 sulfonamides (SAs) in animal feed using high performance liquid chromatography equipped with a photo-diode array detector (HPLC/PDA) and immunoaffinity chromatography was developed. The chromatographic peaks of the 16 SAs were successfully identified by comparing their retention times and UV spectra with reference standards. Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study sulfonamides ranged from 14.1 to 45.0 μg/kg, and the limits of quantification (LOQs) ranged from 46.9 to 150.0 μg/kg. Average recoveries of the 16 SAs ranged from 78.2% to 105.2%. Method replication resulted in intraday and interday peak area variation of <5.5%. The developed method was specific and reliable and is suited for the routine analysis of SAs in animal feed.

AB - A novel and simple method for detecting 16 sulfonamides (SAs) in animal feed using high performance liquid chromatography equipped with a photo-diode array detector (HPLC/PDA) and immunoaffinity chromatography was developed. The chromatographic peaks of the 16 SAs were successfully identified by comparing their retention times and UV spectra with reference standards. Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study sulfonamides ranged from 14.1 to 45.0 μg/kg, and the limits of quantification (LOQs) ranged from 46.9 to 150.0 μg/kg. Average recoveries of the 16 SAs ranged from 78.2% to 105.2%. Method replication resulted in intraday and interday peak area variation of <5.5%. The developed method was specific and reliable and is suited for the routine analysis of SAs in animal feed.

KW - Animal feed

KW - Immunoaffinity chromatography

KW - Sulfonamide

UR - http://www.scopus.com/inward/record.url?scp=84976602948&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2015.10.014

DO - 10.1016/j.foodchem.2015.10.014

M3 - Article

C2 - 26593600

AN - SCOPUS:84976602948

SN - 0308-8146

VL - 196

SP - 1144

EP - 1149

JO - Food Chemistry

JF - Food Chemistry

ER -

Development of an immunoaffinity chromatography and HPLC-UV method for determination of 16 sulfonamides in feed

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this