Development of an SH-SAW sensor for the detection of DNA hybridization

Youngjune Hur; Jinho Han; Jooheon Seon; Yukeun Eugene Pak; Yongrae Roh

doi:10.1016/j.sna.2005.01.027

Development of an SH-SAW sensor for the detection of DNA hybridization

Youngjune Hur, Jinho Han, Jooheon Seon, Yukeun Eugene Pak, Yongrae Roh

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for the detection of DNA (deoxyribonucleic acid) hybridization on the gold-coated delay line of transverse SAW devices. DNA hybridization experiments were performed with 15-mer oligonucleotides (probe and complementary target DNA). The sensor consists of twin SAW delay line oscillators (sensing channel and reference channel) operating at 100 MHz fabricated on 36° rotated Y-cut X-propagation LiTaO₃ piezoelectric single crystals. The relative change in the frequency of the two oscillators was monitored to detect hybridization between the target DNA and probe DNA immobilized on the delay line of the SAW sensor. The measurement results showed a good response of the sensor to the mass loading effects of the DNA hybridization with a sensitivity level up to 1.55 ng/ml/Hz.

Original language	English
Pages (from-to)	462-467
Number of pages	6
Journal	Sensors and Actuators A: Physical
Volume	120
Issue number	2
DOIs	https://doi.org/10.1016/j.sna.2005.01.027
State	Published - 17 May 2005

Keywords

DNA hybridization
DNA immobilization
Oligonucleotide
Oscillator
SH-SAW

Access to Document

10.1016/j.sna.2005.01.027

Cite this

@article{93d3223c54d943aca26f066cb635c178,

title = "Development of an SH-SAW sensor for the detection of DNA hybridization",

abstract = "We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for the detection of DNA (deoxyribonucleic acid) hybridization on the gold-coated delay line of transverse SAW devices. DNA hybridization experiments were performed with 15-mer oligonucleotides (probe and complementary target DNA). The sensor consists of twin SAW delay line oscillators (sensing channel and reference channel) operating at 100 MHz fabricated on 36° rotated Y-cut X-propagation LiTaO3 piezoelectric single crystals. The relative change in the frequency of the two oscillators was monitored to detect hybridization between the target DNA and probe DNA immobilized on the delay line of the SAW sensor. The measurement results showed a good response of the sensor to the mass loading effects of the DNA hybridization with a sensitivity level up to 1.55 ng/ml/Hz.",

keywords = "DNA hybridization, DNA immobilization, Oligonucleotide, Oscillator, SH-SAW",

author = "Youngjune Hur and Jinho Han and Jooheon Seon and Pak, {Yukeun Eugene} and Yongrae Roh",

year = "2005",

month = may,

day = "17",

doi = "10.1016/j.sna.2005.01.027",

language = "English",

volume = "120",

pages = "462--467",

journal = "Sensors and Actuators A: Physical",

issn = "0924-4247",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Development of an SH-SAW sensor for the detection of DNA hybridization

AU - Hur, Youngjune

AU - Han, Jinho

AU - Seon, Jooheon

AU - Pak, Yukeun Eugene

AU - Roh, Yongrae

PY - 2005/5/17

Y1 - 2005/5/17

N2 - We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for the detection of DNA (deoxyribonucleic acid) hybridization on the gold-coated delay line of transverse SAW devices. DNA hybridization experiments were performed with 15-mer oligonucleotides (probe and complementary target DNA). The sensor consists of twin SAW delay line oscillators (sensing channel and reference channel) operating at 100 MHz fabricated on 36° rotated Y-cut X-propagation LiTaO3 piezoelectric single crystals. The relative change in the frequency of the two oscillators was monitored to detect hybridization between the target DNA and probe DNA immobilized on the delay line of the SAW sensor. The measurement results showed a good response of the sensor to the mass loading effects of the DNA hybridization with a sensitivity level up to 1.55 ng/ml/Hz.

AB - We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for the detection of DNA (deoxyribonucleic acid) hybridization on the gold-coated delay line of transverse SAW devices. DNA hybridization experiments were performed with 15-mer oligonucleotides (probe and complementary target DNA). The sensor consists of twin SAW delay line oscillators (sensing channel and reference channel) operating at 100 MHz fabricated on 36° rotated Y-cut X-propagation LiTaO3 piezoelectric single crystals. The relative change in the frequency of the two oscillators was monitored to detect hybridization between the target DNA and probe DNA immobilized on the delay line of the SAW sensor. The measurement results showed a good response of the sensor to the mass loading effects of the DNA hybridization with a sensitivity level up to 1.55 ng/ml/Hz.

KW - DNA hybridization

KW - DNA immobilization

KW - Oligonucleotide

KW - Oscillator

KW - SH-SAW

UR - http://www.scopus.com/inward/record.url?scp=17644414952&partnerID=8YFLogxK

U2 - 10.1016/j.sna.2005.01.027

DO - 10.1016/j.sna.2005.01.027

M3 - Article

AN - SCOPUS:17644414952

SN - 0924-4247

VL - 120

SP - 462

EP - 467

JO - Sensors and Actuators A: Physical

JF - Sensors and Actuators A: Physical

IS - 2

ER -

Development of an SH-SAW sensor for the detection of DNA hybridization

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this