Development of diagnostic PCR system for three seed-transmitted quarantine viruses associated with cucurbitaceae

Siwon Lee; Byung Dae Min; Jin Young Lee; Yong Gil Shin; Su Heon Lee; Weon Hwa Jheong

doi:10.4014/mbl.1410.10008

Development of diagnostic PCR system for three seed-transmitted quarantine viruses associated with cucurbitaceae

Siwon Lee, Byung Dae Min, Jin Young Lee, Yong Gil Shin, Su Heon Lee, Weon Hwa Jheong

Department of Plant Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.

Original language	English
Pages (from-to)	79-83
Number of pages	5
Journal	Microbiology and Biotechnology Letters
Volume	43
Issue number	1
DOIs	https://doi.org/10.4014/mbl.1410.10008
State	Published - 1 Mar 2015

Keywords

CGMMV
Cucurbitaceae
KGMMV
Plant quarantine
SqMV

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title = "Development of diagnostic PCR system for three seed-transmitted quarantine viruses associated with cucurbitaceae",

abstract = "The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.",

keywords = "CGMMV, Cucurbitaceae, KGMMV, Plant quarantine, SqMV",

author = "Siwon Lee and Min, \{Byung Dae\} and Lee, \{Jin Young\} and Shin, \{Yong Gil\} and Lee, \{Su Heon\} and Jheong, \{Weon Hwa\}",

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year = "2015",

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AU - Min, Byung Dae

AU - Lee, Jin Young

AU - Shin, Yong Gil

AU - Lee, Su Heon

AU - Jheong, Weon Hwa

PY - 2015/3/1

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N2 - The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.

AB - The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.

KW - CGMMV

KW - Cucurbitaceae

KW - KGMMV

KW - Plant quarantine

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Development of diagnostic PCR system for three seed-transmitted quarantine viruses associated with cucurbitaceae

Abstract

Keywords

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