TY - JOUR
T1 - Development of EST-SSR Markers and Analysis of Genetic Relationship it’s Resources in Hexaploid Oats
AU - Isabel, Leitao Pedro
AU - Park, Jae Ryoung
AU - Lee, Gang Seob
AU - Park, Gyu Hwan
AU - Kim, Kyung Min
N1 - Publisher Copyright:
© 2019, Korean Society of Crop Science and Springer.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Oats are the fifth largest planted area in the world, and are the only cereal crops in the world to be among the top ten in the world. However, since oats are weak in cold weather, only four varieties are cultivated in Korea at present. Therefore, we constructed the DNA profile database using the EST-SSR marker for the oat 73 varieties kept in Korea. When the oat 73 cultivars marketed in Korea were tested with the eight EST-SSR marker developed in this study, 71.62 average alleles were detected per marker and the mean of PIC value was 0.337. According to the polymorphism of microsatellite markers, 73 varieties of oats were classified into 5 ~ 9 groups and most of the genetic resources investigated were identified when EST-SSR marker was used. In the future, EST-SSR markers developed in this study may be used to identify the seed disputes and breeds of oats. In addition, it predicts that it will be usefully used as a means of evaluating the characteristics of oat genetic resources, testing purity and studying the cultivation of varieties.
AB - Oats are the fifth largest planted area in the world, and are the only cereal crops in the world to be among the top ten in the world. However, since oats are weak in cold weather, only four varieties are cultivated in Korea at present. Therefore, we constructed the DNA profile database using the EST-SSR marker for the oat 73 varieties kept in Korea. When the oat 73 cultivars marketed in Korea were tested with the eight EST-SSR marker developed in this study, 71.62 average alleles were detected per marker and the mean of PIC value was 0.337. According to the polymorphism of microsatellite markers, 73 varieties of oats were classified into 5 ~ 9 groups and most of the genetic resources investigated were identified when EST-SSR marker was used. In the future, EST-SSR markers developed in this study may be used to identify the seed disputes and breeds of oats. In addition, it predicts that it will be usefully used as a means of evaluating the characteristics of oat genetic resources, testing purity and studying the cultivation of varieties.
KW - Cereal crop
KW - EST (Expressed Sequence Tags)
KW - Oat
KW - SSR (Simple Sequence Repeats)
KW - Seed dispute
UR - http://www.scopus.com/inward/record.url?scp=85072970084&partnerID=8YFLogxK
U2 - 10.1007/s12892-019-0158-0
DO - 10.1007/s12892-019-0158-0
M3 - Article
AN - SCOPUS:85072970084
SN - 1975-9479
VL - 22
SP - 243
EP - 251
JO - Journal of Crop Science and Biotechnology
JF - Journal of Crop Science and Biotechnology
IS - 3
ER -