Development of one-step real-time reverse transcription-polymerase chain reaction in combination with automated RNA extraction for detection and quantitation of hepatitis A virus

Byoung Guk Kim; Hye Sung Jeong; Sun Young Baek; Jin Ho Shin; Jae Ok Kim; Kyung Il Min; Seung Rel Ryu; Bok Soon Min; Do Keun Kim; Mi Kyung Park; Mi Jin Ahn; Seok Ho Lee; Sue Nie Park

Development of one-step real-time reverse transcription-polymerase chain reaction in combination with automated RNA extraction for detection and quantitation of hepatitis A virus

Byoung Guk Kim, Hye Sung Jeong, Sun Young Baek, Jin Ho Shin, Jae Ok Kim, Kyung Il Min, Seung Rel Ryu, Bok Soon Min, Do Keun Kim, Mi Kyung Park, Mi Jin Ahn, Seok Ho Lee, Sue Nie Park

School of Food Science & Biotechnology

Food and Drug Administration of Korea

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

One-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using the MagNA Pure LC and LightCycler™ system was developed and validated for the detection and quantitation of hepatitis A virus (HAV) RNA. The assay was evaluated using in-house synthetic HAV RNA standard. The real-time RT-PCR assay could quantitate a dynamic range of HAV RNA standard between 10² and 10⁸ copies per reaction. The regression coefficient of the standard curve was an 0.99. The detection limit of the assay was 31.3 RNA copies per reaction. The coefficient variations (CVs) of the assay in combination with automated RNA extraction were less than 1.91% in both intra- and inter-assay. The real-time RT-PCR assay for quantitative detection of HAV would serve a useful method for improving the safety of biological products.

Original language	English
Pages (from-to)	209-218
Number of pages	10
Journal	Journal of Bacteriology and Virology
Volume	33
Issue number	3
State	Published - 2003

Keywords

HAV
Quantitation
Real-time RT-PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Kim, B. G., Jeong, H. S., Baek, S. Y., Shin, J. H., Kim, J. O., Min, K. I., Ryu, S. R., Min, B. S., Kim, D. K., Park, M. K., Ahn, M. J., Lee, S. H., & Park, S. N. (2003). Development of one-step real-time reverse transcription-polymerase chain reaction in combination with automated RNA extraction for detection and quantitation of hepatitis A virus. Journal of Bacteriology and Virology, 33(3), 209-218.

@article{74bb53b1ded149d7b0763b883b371a84,

title = "Development of one-step real-time reverse transcription-polymerase chain reaction in combination with automated RNA extraction for detection and quantitation of hepatitis A virus",

abstract = "One-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using the MagNA Pure LC and LightCycler{\texttrademark} system was developed and validated for the detection and quantitation of hepatitis A virus (HAV) RNA. The assay was evaluated using in-house synthetic HAV RNA standard. The real-time RT-PCR assay could quantitate a dynamic range of HAV RNA standard between 102 and 108 copies per reaction. The regression coefficient of the standard curve was an 0.99. The detection limit of the assay was 31.3 RNA copies per reaction. The coefficient variations (CVs) of the assay in combination with automated RNA extraction were less than 1.91% in both intra- and inter-assay. The real-time RT-PCR assay for quantitative detection of HAV would serve a useful method for improving the safety of biological products.",

keywords = "HAV, Quantitation, Real-time RT-PCR",

author = "Kim, {Byoung Guk} and Jeong, {Hye Sung} and Baek, {Sun Young} and Shin, {Jin Ho} and Kim, {Jae Ok} and Min, {Kyung Il} and Ryu, {Seung Rel} and Min, {Bok Soon} and Kim, {Do Keun} and Park, {Mi Kyung} and Ahn, {Mi Jin} and Lee, {Seok Ho} and Park, {Sue Nie}",

year = "2003",

language = "English",

volume = "33",

pages = "209--218",

journal = "Journal of Bacteriology and Virology",

issn = "1598-2467",

publisher = "The Korean Society for Mocrobiology / The Korean Society of Virology",

number = "3",

}

Kim, BG, Jeong, HS, Baek, SY, Shin, JH, Kim, JO, Min, KI, Ryu, SR, Min, BS, Kim, DK, Park, MK, Ahn, MJ, Lee, SH & Park, SN 2003, 'Development of one-step real-time reverse transcription-polymerase chain reaction in combination with automated RNA extraction for detection and quantitation of hepatitis A virus', Journal of Bacteriology and Virology, vol. 33, no. 3, pp. 209-218.

Development of one-step real-time reverse transcription-polymerase chain reaction in combination with automated RNA extraction for detection and quantitation of hepatitis A virus. / Kim, Byoung Guk; Jeong, Hye Sung; Baek, Sun Young et al.
In: Journal of Bacteriology and Virology, Vol. 33, No. 3, 2003, p. 209-218.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development of one-step real-time reverse transcription-polymerase chain reaction in combination with automated RNA extraction for detection and quantitation of hepatitis A virus

AU - Kim, Byoung Guk

AU - Jeong, Hye Sung

AU - Baek, Sun Young

AU - Shin, Jin Ho

AU - Kim, Jae Ok

AU - Min, Kyung Il

AU - Ryu, Seung Rel

AU - Min, Bok Soon

AU - Kim, Do Keun

AU - Park, Mi Kyung

AU - Ahn, Mi Jin

AU - Lee, Seok Ho

AU - Park, Sue Nie

PY - 2003

Y1 - 2003

N2 - One-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using the MagNA Pure LC and LightCycler™ system was developed and validated for the detection and quantitation of hepatitis A virus (HAV) RNA. The assay was evaluated using in-house synthetic HAV RNA standard. The real-time RT-PCR assay could quantitate a dynamic range of HAV RNA standard between 102 and 108 copies per reaction. The regression coefficient of the standard curve was an 0.99. The detection limit of the assay was 31.3 RNA copies per reaction. The coefficient variations (CVs) of the assay in combination with automated RNA extraction were less than 1.91% in both intra- and inter-assay. The real-time RT-PCR assay for quantitative detection of HAV would serve a useful method for improving the safety of biological products.

AB - One-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using the MagNA Pure LC and LightCycler™ system was developed and validated for the detection and quantitation of hepatitis A virus (HAV) RNA. The assay was evaluated using in-house synthetic HAV RNA standard. The real-time RT-PCR assay could quantitate a dynamic range of HAV RNA standard between 102 and 108 copies per reaction. The regression coefficient of the standard curve was an 0.99. The detection limit of the assay was 31.3 RNA copies per reaction. The coefficient variations (CVs) of the assay in combination with automated RNA extraction were less than 1.91% in both intra- and inter-assay. The real-time RT-PCR assay for quantitative detection of HAV would serve a useful method for improving the safety of biological products.

KW - HAV

KW - Quantitation

KW - Real-time RT-PCR

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M3 - Article

AN - SCOPUS:24044446556

SN - 1598-2467

VL - 33

SP - 209

EP - 218

JO - Journal of Bacteriology and Virology

JF - Journal of Bacteriology and Virology

IS - 3

ER -

Development of one-step real-time reverse transcription-polymerase chain reaction in combination with automated RNA extraction for detection and quantitation of hepatitis A virus

Abstract

Keywords

UN SDGs

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