Abstract
We developed new surface acoustic wave (SAW) sensors to measure the properties of protein solutions applying a particular organic thin film on the delay line of transverse type SAW devices. Each delay line is configured as an oscillator. The delay line for a sensing channel is coated with a gold film on which an antibody layer is immobilized by protein A. The sensing delay line selectively adsorbs antigens when exposed to a protein solution, which results in phase delay changes due to the mass loading effects induced by the adsorbed antigens. The other delay line is uncoated for use as a stable reference. The relative change in the frequency of the two oscillators is monitored to measure the concentration of the antigens. Sensor properties investigated include selectivity, sensitivity, response time and stability in response to the antigen concentration as well as the viscosity and electrical conductivity of the protein solution.
| Original language | English |
|---|---|
| Pages (from-to) | 98-105 |
| Number of pages | 8 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 5055 |
| DOIs | |
| State | Published - 2003 |
| Event | Smart Structures and Materials 2003: Smart Electronics, MEMS, BioMEMS, and Nanotechnology - San Diego, CA, United States Duration: 3 Mar 2003 → 5 Mar 2003 |
Keywords
- LiTaO3 single crystals
- Oscillator
- Protein
- SH-SAW sensor