Diapause-regulated proteins in the gut of pharate first instar larvae of the gypsy moth, Lymantria dispar, and the effect of KK-42 and neck ligation on expression

Kyeong Yeoll Lee, David L. Denlinger

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Two groups of proteins in the gut of the gypsy moth, Lymantria dispar, have distinct expression patterns in relation to diapause. Two proteins, 30 and 35 kDa, are highly expressed prior to the inception of diapause (13-17 days after oviposition) and then cease to be expressed shortly thereafter. Two other proteins, 55 and 60 kDa, are highly expressed in early diapause and then gradually decline in expression as diapause progresses. Expression of the 55 kDa protein ceases during the chilling period required to terminate diapause while expression of the 60 kDa protein continues even in postdiapause. Additional experiments focused on the 55 kDa protein due to its expression being restricted to early diapause. The 55 kDa protein is gut specific and within the gut it is expressed in only the mid- and hindgut. KK-42, an imidazole derivative known to avert diapause in the gypsy moth, prevents expression of the 55 kDa protein. Neck ligation experiments indicate that the brain is not needed to stimulate synthesis of the protein. The protein, however, is not synthesized when the gut is cultured in vitro. This suggests that some tissue other than the brain and external to the gut is needed to prompt synthesis of the 55 kDa protein.

Original languageEnglish
Pages (from-to)423-431
Number of pages9
JournalJournal of Insect Physiology
Volume42
Issue number5
DOIs
StatePublished - 1996

Keywords

  • Brain
  • Diapause regulation
  • Gut proteins
  • KK-42
  • Lymantria dispar

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