Differential expression of transforming growth factor-β in the interstitial tissue of testis during aging

Jae Chang Jung; Geun Tae Park; Kook Hee Kim; Ju Hyung Woo; Jung Min An; Ki Chul Kim; Hae Young Chung; Young Seuk Bae; Jeen Woo Park; Shin Sung Kang; Young Sup Lee

doi:10.1002/jcb.20042

Differential expression of transforming growth factor-β in the interstitial tissue of testis during aging

Jae Chang Jung, Geun Tae Park, Kook Hee Kim, Ju Hyung Woo, Jung Min An, Ki Chul Kim, Hae Young Chung, Young Seuk Bae, Jeen Woo Park, Shin Sung Kang, Young Sup Lee

Department of Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Transforming growth factor-βs (TGF-βs) have significant effects on testis development. The pattern of TGF-β expression in aging testis has not been established to date. We examined age-related changes in the expression of TGF-β and its receptors in the testis using Western blot analysis. TGF-β1 expression increased continuously in aging rat testis, whereas no age-associated changes were observed for TGF-β3. Strong expression of TGF-β2, as well as type I and II receptors was observed in 12-month-old testis, but following this time, expression decreased dramatically. Interestingly, TGF-β2 and -β3 displayed strong and similar expression patterns in liver, regardless of age, suggesting that the down-regulation of TGF-β2 is testis-specific. We observed significant induction of p53 and p21^WAF1 in 18-month-old testis that appeared to correspond with aging. Moreover, caloric restriction (CR) prevented age-related decrease in TGF-β2 expression. Using immunohistochemistry, we showed that all TGF-β1, -β2, and -β3 proteins are expressed primarily in interstitial cells, which are located in the space between adjoining seminiferous tubules. Our data collectively indicate that aging in the testis is regulated by differential expression of TGF-β proteins, and decreased levels of TGF-β2 contribute to the aging process.

Original language	English
Pages (from-to)	92-98
Number of pages	7
Journal	Journal of Cellular Biochemistry
Volume	92
Issue number	1
DOIs	https://doi.org/10.1002/jcb.20042
State	Published - 2004

Keywords

Senescence
Testis
TGF-β

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10.1002/jcb.20042

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title = "Differential expression of transforming growth factor-β in the interstitial tissue of testis during aging",

abstract = "Transforming growth factor-βs (TGF-βs) have significant effects on testis development. The pattern of TGF-β expression in aging testis has not been established to date. We examined age-related changes in the expression of TGF-β and its receptors in the testis using Western blot analysis. TGF-β1 expression increased continuously in aging rat testis, whereas no age-associated changes were observed for TGF-β3. Strong expression of TGF-β2, as well as type I and II receptors was observed in 12-month-old testis, but following this time, expression decreased dramatically. Interestingly, TGF-β2 and -β3 displayed strong and similar expression patterns in liver, regardless of age, suggesting that the down-regulation of TGF-β2 is testis-specific. We observed significant induction of p53 and p21WAF1 in 18-month-old testis that appeared to correspond with aging. Moreover, caloric restriction (CR) prevented age-related decrease in TGF-β2 expression. Using immunohistochemistry, we showed that all TGF-β1, -β2, and -β3 proteins are expressed primarily in interstitial cells, which are located in the space between adjoining seminiferous tubules. Our data collectively indicate that aging in the testis is regulated by differential expression of TGF-β proteins, and decreased levels of TGF-β2 contribute to the aging process.",

keywords = "Senescence, Testis, TGF-β",

author = "Jung, {Jae Chang} and Park, {Geun Tae} and Kim, {Kook Hee} and Woo, {Ju Hyung} and An, {Jung Min} and Kim, {Ki Chul} and Chung, {Hae Young} and Bae, {Young Seuk} and Park, {Jeen Woo} and Kang, {Shin Sung} and Lee, {Young Sup}",

year = "2004",

doi = "10.1002/jcb.20042",

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journal = "Journal of Cellular Biochemistry",

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AU - Jung, Jae Chang

AU - Park, Geun Tae

AU - Kim, Kook Hee

AU - Woo, Ju Hyung

AU - An, Jung Min

AU - Kim, Ki Chul

AU - Chung, Hae Young

AU - Bae, Young Seuk

AU - Park, Jeen Woo

AU - Kang, Shin Sung

AU - Lee, Young Sup

PY - 2004

Y1 - 2004

N2 - Transforming growth factor-βs (TGF-βs) have significant effects on testis development. The pattern of TGF-β expression in aging testis has not been established to date. We examined age-related changes in the expression of TGF-β and its receptors in the testis using Western blot analysis. TGF-β1 expression increased continuously in aging rat testis, whereas no age-associated changes were observed for TGF-β3. Strong expression of TGF-β2, as well as type I and II receptors was observed in 12-month-old testis, but following this time, expression decreased dramatically. Interestingly, TGF-β2 and -β3 displayed strong and similar expression patterns in liver, regardless of age, suggesting that the down-regulation of TGF-β2 is testis-specific. We observed significant induction of p53 and p21WAF1 in 18-month-old testis that appeared to correspond with aging. Moreover, caloric restriction (CR) prevented age-related decrease in TGF-β2 expression. Using immunohistochemistry, we showed that all TGF-β1, -β2, and -β3 proteins are expressed primarily in interstitial cells, which are located in the space between adjoining seminiferous tubules. Our data collectively indicate that aging in the testis is regulated by differential expression of TGF-β proteins, and decreased levels of TGF-β2 contribute to the aging process.

AB - Transforming growth factor-βs (TGF-βs) have significant effects on testis development. The pattern of TGF-β expression in aging testis has not been established to date. We examined age-related changes in the expression of TGF-β and its receptors in the testis using Western blot analysis. TGF-β1 expression increased continuously in aging rat testis, whereas no age-associated changes were observed for TGF-β3. Strong expression of TGF-β2, as well as type I and II receptors was observed in 12-month-old testis, but following this time, expression decreased dramatically. Interestingly, TGF-β2 and -β3 displayed strong and similar expression patterns in liver, regardless of age, suggesting that the down-regulation of TGF-β2 is testis-specific. We observed significant induction of p53 and p21WAF1 in 18-month-old testis that appeared to correspond with aging. Moreover, caloric restriction (CR) prevented age-related decrease in TGF-β2 expression. Using immunohistochemistry, we showed that all TGF-β1, -β2, and -β3 proteins are expressed primarily in interstitial cells, which are located in the space between adjoining seminiferous tubules. Our data collectively indicate that aging in the testis is regulated by differential expression of TGF-β proteins, and decreased levels of TGF-β2 contribute to the aging process.

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Differential expression of transforming growth factor-β in the interstitial tissue of testis during aging

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