Differential functions of genes regulated by VEGF-NFATc1 signaling pathway in the migration of pulmonary valve endothelial cells

Gun Hyuk Jang, In Sook Park, Jeong Hee Yang, Joyce Bischoff, You Mie Lee

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We have reported that vascular endothelial growth factor (VEGF)-A induces the proliferation of human pulmonary valve endothelial cells (HPVECs) through nuclear factor in activated T cells (NFAT)c1 activation [1]. Here we show that VEGF-A increases the migration of HPVECs through NFATc1 activation, suggesting that VEGF-A/NFATc1 regulates the migration of HPVECs. To learn how this pathway may be involved in post-natal valvular repair, HPVECs were treated with VEGF-A, with or without cyclosporine A to selectively block VEGF-NFATc1 signaling. Down Syndrome critical region 1 (DSCR1) and heparin-binding EGF-like growth factor (HB-EGF) are two genes identified by DNA microarray as being up-regulated by VEGF-A in a cyclosporine-A-sensitive manner. DSCR1 silencing increased the migration of ovine valve endothelial cells, whereas HB-EGF silencing inhibited migration. This differential effect suggests that VEGF-A/NFATc1 signaling might be a crucial coordinator of endothelial cell migration in post-natal valves.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalFEBS Letters
Volume584
Issue number1
DOIs
StatePublished - 4 Jan 2010

Keywords

  • Calcineurin
  • Down Syndrome critical region 1
  • Endothelial-mesenchymal transdifferentiation
  • Heparin-binding EGF-like growth factor
  • Nuclear factor in activated T cells c1
  • Valve endothelial cells
  • Vascular endothelial growth factor

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