Differential pharmacological properties of GABA_A receptors in axon terminals and soma of dentate gyrus granule cells

Although it has been well established that GABA_A receptors are molecular targets of a variety of allosteric modulators, such as benzodiazepines, the pharmacological properties of presynaptic GABA_A receptors are poorly understood. In this study, the effects of diazepam and Zn²⁺ on presynaptic GABA_A receptors have been investigated by measuring the GABA_A receptor-mediated facilitation of spontaneous glutamate release in mechanically dissociated rat CA3 pyramidal neurons. Diazepam significantly enhanced the muscimol-induced facilitation (particularly at submicromolar concentrations) of spontaneous glutamate release and shifted the concentration-response relationship for muscimol toward the left, whereas Zn²⁺ (≤ 100 μM) had little effect on the muscimol-induced facilitation of spontaneous glutamate release. In contrast, Zn²⁺ significantly suppressed the muscimol-induced currents mediated by GABA _A receptors expressed on dentate gyrus granule cells, which are parent neurons of mossy fibers, whereas the effect of diazepam on GABA _A receptors expressed on dentate gyrus granule cells was lesser than that on presynaptic GABA_A receptors. The results suggest that the pharmacological properties of GABA_A receptors differ considerably between presynaptic (axon terminals) and somatic regions in the same granule cell and that presynaptic GABA_A receptors should be considered as one of the important pharmacological targets of many drugs affecting GABA _A receptors.