Abstract
Ordered differential display PCR was used to identify differentially expressed genes in rat dorsal root ganglia at 7 days following chronic constriction injury (CCI) of the sciatic nerve. Fourteen differentially displayed cDNA bands were isolated, cloned and verified by RT-PCR. The four mRNAs were increased, which included mRNAs encoding heat shock protein 27, fatty acid binding protein, apolipoprotein D and one novel gene. Six down-regulated clones were microtubule-associated protein IB, protein tyrosine phosphatase α, Kvl.2 channel, myelin protein SR13, medium-sized neurofilament protein, and one novel gene. Our results show that many differentially regulated genes after CCI may play a role in nerve degeneration and/or regeneration and provide a molecular framework for understanding the peripheral mechanism underlying neuropathic pain.
Original language | English |
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Pages (from-to) | 3401-3405 |
Number of pages | 5 |
Journal | NeuroReport |
Volume | 12 |
Issue number | 15 |
DOIs | |
State | Published - 29 Oct 2001 |
Keywords
- Chronic constriction injury
- Dorsal root ganglia
- Neuropathic pain
- Ordered differential display-polymerase chain reaction