Differentially expressed genes in rat dorsal root ganglia following peripheral nerve injury

Dong Sun Kim, Sang Ji Lee, So Yun Park, Hea Jin Yoo, Shin Hee Kim, Kwang Jin Kim, Hee Jung Cho

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Ordered differential display PCR was used to identify differentially expressed genes in rat dorsal root ganglia at 7 days following chronic constriction injury (CCI) of the sciatic nerve. Fourteen differentially displayed cDNA bands were isolated, cloned and verified by RT-PCR. The four mRNAs were increased, which included mRNAs encoding heat shock protein 27, fatty acid binding protein, apolipoprotein D and one novel gene. Six down-regulated clones were microtubule-associated protein IB, protein tyrosine phosphatase α, Kvl.2 channel, myelin protein SR13, medium-sized neurofilament protein, and one novel gene. Our results show that many differentially regulated genes after CCI may play a role in nerve degeneration and/or regeneration and provide a molecular framework for understanding the peripheral mechanism underlying neuropathic pain.

Original languageEnglish
Pages (from-to)3401-3405
Number of pages5
JournalNeuroReport
Volume12
Issue number15
DOIs
StatePublished - 29 Oct 2001

Keywords

  • Chronic constriction injury
  • Dorsal root ganglia
  • Neuropathic pain
  • Ordered differential display-polymerase chain reaction

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