Diversity, pathogenicity, and fungicide sensitivity of colletotrichum species associated with apple anthracnose in South Korea

Apple fruits with anthracnose symptoms were collected from commercial study also reidentified six Colletotrichum isolates responsible for apple apple orchaffis in different regions of the Republic of Korea, and isolations anthracnose, which were deposited in the Korean Agricultural Culture were made on potato dextmse agar to isolate the causal agents. The fungal Collection. Among the six isolates, three were identified as C.siamense isolates were identified based on their morphological characteristics, (deposited as C. gloeosporioides s.s.), and three were C. nymphaeae gmwth rates, and multigene sequences. Nine isolates were identified via (deposited as C. acutatum s.s.). All the Colletotrichum species identi phylogeneti analysis: three Colletotrichum fructicola, two C. fioriniae, fied in this study were highly sensitive to tebuconazole in terms of in- one C. gloeosporioides sensu stricto (s.s.), two C. nymphaeae, and one hibition of mycelial growth (EC50 value of 0.12 to 2.1 jig/ml). C. siamense isolates. The pathogenicity of the Colletotrichum isolates was tested using detached apple fruits under laboratory conditions.