Drying effect on enzymatic hydrolysis of cellulose associated with porosity and crystallinity

Bonwook Koo, Jaemin Jo, Seong Min Cho

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12 Scopus citations

Abstract

The effect of drying on the enzymatic hydrolysis of cellulose was determined by analysis of porosity and crystallinity. Fiber hornification induced by drying produced an irreversible reduction in pore volume due to shrinkage and pore collapse, and the decrease in porosity inhibited enzymatic hydrolysis. The drying effect index (DEI) was defined as the difference in enzymatic digestibility between oven-and never-dried pulp, and it was determined that more enzymes caused a higher DEI at the initial stage of enzymatic hydrolysis and the highest DEI was also observed at the earlier stages with higher enzyme dosage. However, there was no significant difference in the DEI with less enzymes because cellulose conversion to sugars during hydrolysis did not enhance enzymatic hydrolysis due to the decrease in enzyme activity. The water retention value (WRV) and Simons' staining were used to measure pore volume and to investigate the cause of the decrease in enzymatic hydrolysis. A decrease in enzyme accessibility induced by the collapse of enzymes' accessible larger pores was determined and this decreased the enzymatic hydrolysis. However, drying once did not cause any irreversible change in the crystalline structure, thus it seems there is no correlation between enzymatic digestibility and crystalline structure.

Original languageEnglish
Article number5545
JournalApplied Sciences (Switzerland)
Volume10
Issue number16
DOIs
StatePublished - Aug 2020

Keywords

  • Cellulose
  • Drying effect
  • Enzymatic hydrolysis
  • Hornification
  • Porosity

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