Effect of a β-glucan from Aureobasidium on TGF-β1-modulated in vitro dermal wound repair

Jae Suk Choi; Joo Wan Kim; Go Woon Jung; Seung Bae Moon; Hyung Rae Cho; Soo Hyun Sung; Jai Jun Jung; Young Sam Kwon; Sae Kwang Ku; Jae Hak Sohn

doi:10.1007/s13530-016-0257-1

Effect of a β-glucan from Aureobasidium on TGF-β₁-modulated in vitro dermal wound repair

Jae Suk Choi, Joo Wan Kim, Go Woon Jung, Seung Bae Moon, Hyung Rae Cho, Soo Hyun Sung, Jai Jun Jung, Young Sam Kwon, Sae Kwang Ku, Jae Hak Sohn

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The objective of the present study was to determine if Aureobasidium-originated beta-glucan (beta-glucan) modulated transforming growth factor (TGF)-β₁-mediated wound healing. Proliferation of and collagen production by human dermal fibroblast cells were measured during in vitro dermal wound repopulation after treatment with 100, 10, 1, 0.1 and 0.01 mg/mL β-glucan and 1 ng/mL TGF-β₁. Control group was treated without β-glucan or 1 ng/mL TGF-β₁. TGF-β₁ significantly decreased the optical density at A₅₇₀ (a measure of fibroblast cell number) and increased procollagen production compared with the control. Also, fibroblast migration into wound defects decreased. The reductions in fibroblast proliferation and migration were significantly and dose-dependently inhibited by β-glucan (at 0.1 mg/mL or higher). However, glucan did not affect procollagen production. Thus, β-glucan may aid wound healing mediated by TGF-β₁, an important cytokine in this context.

Original language	English
Pages (from-to)	12-18
Number of pages	7
Journal	Toxicology and Environmental Health Sciences
Volume	8
Issue number	1
DOIs	https://doi.org/10.1007/s13530-016-0257-1
State	Published - 1 Mar 2016

Keywords

Fibroblast
TGF-β
Wound healing
in vitro
β-glucan

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abstract = "The objective of the present study was to determine if Aureobasidium-originated beta-glucan (beta-glucan) modulated transforming growth factor (TGF)-β1-mediated wound healing. Proliferation of and collagen production by human dermal fibroblast cells were measured during in vitro dermal wound repopulation after treatment with 100, 10, 1, 0.1 and 0.01 mg/mL β-glucan and 1 ng/mL TGF-β1. Control group was treated without β-glucan or 1 ng/mL TGF-β1. TGF-β1 significantly decreased the optical density at A570 (a measure of fibroblast cell number) and increased procollagen production compared with the control. Also, fibroblast migration into wound defects decreased. The reductions in fibroblast proliferation and migration were significantly and dose-dependently inhibited by β-glucan (at 0.1 mg/mL or higher). However, glucan did not affect procollagen production. Thus, β-glucan may aid wound healing mediated by TGF-β1, an important cytokine in this context.",

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AU - Jung, Go Woon

AU - Moon, Seung Bae

AU - Cho, Hyung Rae

AU - Sung, Soo Hyun

AU - Jung, Jai Jun

AU - Kwon, Young Sam

AU - Ku, Sae Kwang

AU - Sohn, Jae Hak

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N2 - The objective of the present study was to determine if Aureobasidium-originated beta-glucan (beta-glucan) modulated transforming growth factor (TGF)-β1-mediated wound healing. Proliferation of and collagen production by human dermal fibroblast cells were measured during in vitro dermal wound repopulation after treatment with 100, 10, 1, 0.1 and 0.01 mg/mL β-glucan and 1 ng/mL TGF-β1. Control group was treated without β-glucan or 1 ng/mL TGF-β1. TGF-β1 significantly decreased the optical density at A570 (a measure of fibroblast cell number) and increased procollagen production compared with the control. Also, fibroblast migration into wound defects decreased. The reductions in fibroblast proliferation and migration were significantly and dose-dependently inhibited by β-glucan (at 0.1 mg/mL or higher). However, glucan did not affect procollagen production. Thus, β-glucan may aid wound healing mediated by TGF-β1, an important cytokine in this context.

AB - The objective of the present study was to determine if Aureobasidium-originated beta-glucan (beta-glucan) modulated transforming growth factor (TGF)-β1-mediated wound healing. Proliferation of and collagen production by human dermal fibroblast cells were measured during in vitro dermal wound repopulation after treatment with 100, 10, 1, 0.1 and 0.01 mg/mL β-glucan and 1 ng/mL TGF-β1. Control group was treated without β-glucan or 1 ng/mL TGF-β1. TGF-β1 significantly decreased the optical density at A570 (a measure of fibroblast cell number) and increased procollagen production compared with the control. Also, fibroblast migration into wound defects decreased. The reductions in fibroblast proliferation and migration were significantly and dose-dependently inhibited by β-glucan (at 0.1 mg/mL or higher). However, glucan did not affect procollagen production. Thus, β-glucan may aid wound healing mediated by TGF-β1, an important cytokine in this context.

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Effect of a β-glucan from Aureobasidium on TGF-β₁-modulated in vitro dermal wound repair

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Keywords

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