TY - JOUR
T1 - Effect of high temperature on bacterial community dynamics in anaerobic acidogenesis using mesophilic sludge inoculum
AU - Kim, Woong
AU - Hwang, Kwanghyun
AU - Shin, Seung Gu
AU - Lee, Seungyong
AU - Hwang, Seokhwan
PY - 2010/1
Y1 - 2010/1
N2 - In this study, we investigated the microbial community dynamics in thermal acidogenesis using mesophilic sludge. From the result of optimization with a response surface methodology, the acidogenic optimum conditions predicted were a hydraulic retention time of 2.0 days and 51 °C. Denaturing gradient gel electrophoresis (DGGE) profiles shows that the monitored bacterial community present consists of Pseudomonas mendocina, Bacillus halodurans, Clostridium hastiforme, Gracilibacter thermotolerans, and Thermomonas haemolytica. Among these, B. halodurans, G. thermotolerans, and T. haemolytica are reported to ferment carbohydrates thermotolerantly. In contrast, P. mendocina disappeared in the acidogenesis process because of its mesophilicity. In addition, C. hastiforme, G. thermotolerans originating from mesophilic anaerobic sludge were detected in the thermal acidogenesis. Based on this finding, we inferred that most thermophiles detected as DGGE bands could grow catalyzing carbohydrates metabolism in swine wastewater to produce volatile fatty acids thermotolerantly.
AB - In this study, we investigated the microbial community dynamics in thermal acidogenesis using mesophilic sludge. From the result of optimization with a response surface methodology, the acidogenic optimum conditions predicted were a hydraulic retention time of 2.0 days and 51 °C. Denaturing gradient gel electrophoresis (DGGE) profiles shows that the monitored bacterial community present consists of Pseudomonas mendocina, Bacillus halodurans, Clostridium hastiforme, Gracilibacter thermotolerans, and Thermomonas haemolytica. Among these, B. halodurans, G. thermotolerans, and T. haemolytica are reported to ferment carbohydrates thermotolerantly. In contrast, P. mendocina disappeared in the acidogenesis process because of its mesophilicity. In addition, C. hastiforme, G. thermotolerans originating from mesophilic anaerobic sludge were detected in the thermal acidogenesis. Based on this finding, we inferred that most thermophiles detected as DGGE bands could grow catalyzing carbohydrates metabolism in swine wastewater to produce volatile fatty acids thermotolerantly.
KW - Acidogen
KW - Anaerobic digestion
KW - Hydraulic retention time
KW - Response surface methodology
KW - Thermophilic
UR - http://www.scopus.com/inward/record.url?scp=73949124538&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2009.03.029
DO - 10.1016/j.biortech.2009.03.029
M3 - Article
C2 - 19362824
AN - SCOPUS:73949124538
SN - 0960-8524
VL - 101
SP - S17-S22
JO - Bioresource Technology
JF - Bioresource Technology
IS - SUPPL.1
ER -
