TY - JOUR
T1 - Effect of mating period and time-of-day for bloodmeal on rearing of Asian tiger mosquito (Aedes albopictus) in laboratory conditions
AU - Kim, Domin
AU - Kim, Donghun
N1 - Publisher Copyright:
© 2020 The Entomological Society of Korea and John Wiley & Sons Australia, Ltd
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Colonization and maintenance of mosquitoes in the laboratory is required to study physiology, ecology, and behavior of mosquitoes and interactions between mosquito and pathogens. Artificial blood feeding systems have been widely used to maintain the laboratory colony of Aedes albopictus. In this study, we investigated the effects of mating period (1, 3, 6, and 10 days) and time-of-day for bloodmeal (08:00, 13:00, and 18:00) in the use of an artificial feeding system on blood-feeding rate, female fecundity, egg hatching rate, and developmental time of the Asian tiger mosquito, A. albopictus. Younger females mated for three or fewer days reproduced more eggs compared to those of oldest females mated for ten days. Similar to the result for eggs laid, the mean egg-hatching rate was significantly higher from the offspring of younger females than from those of older females. However, mating period and time-of-day for bloodmeal had no effect on the blood feeding rate and developmental time. Taken together, we suggest that three-day mating with bloodmeal at 18:00 is optimal for maintaining colonies of A. albopictus in laboratory conditions.
AB - Colonization and maintenance of mosquitoes in the laboratory is required to study physiology, ecology, and behavior of mosquitoes and interactions between mosquito and pathogens. Artificial blood feeding systems have been widely used to maintain the laboratory colony of Aedes albopictus. In this study, we investigated the effects of mating period (1, 3, 6, and 10 days) and time-of-day for bloodmeal (08:00, 13:00, and 18:00) in the use of an artificial feeding system on blood-feeding rate, female fecundity, egg hatching rate, and developmental time of the Asian tiger mosquito, A. albopictus. Younger females mated for three or fewer days reproduced more eggs compared to those of oldest females mated for ten days. Similar to the result for eggs laid, the mean egg-hatching rate was significantly higher from the offspring of younger females than from those of older females. However, mating period and time-of-day for bloodmeal had no effect on the blood feeding rate and developmental time. Taken together, we suggest that three-day mating with bloodmeal at 18:00 is optimal for maintaining colonies of A. albopictus in laboratory conditions.
KW - Aedes albopictus
KW - artificial blood-feeding
KW - mating time
UR - http://www.scopus.com/inward/record.url?scp=85085486118&partnerID=8YFLogxK
U2 - 10.1111/1748-5967.12430
DO - 10.1111/1748-5967.12430
M3 - Article
AN - SCOPUS:85085486118
SN - 1748-5967
VL - 50
SP - 229
EP - 235
JO - Entomological Research
JF - Entomological Research
IS - 5
ER -