Effect of peroxiredoxin II on the quality and mitochondrial activity of pre-implantation bovine embryos

Md Fakruzzaman, Nasser Ghanem, Jae Il Bang, A. Na Ha, Kyeong Lim Lee, Sea Hwan Sohn, Zhongde Wang, Dong Seok Lee, Il Keun Kong

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Endogenous peroxiredoxin II (PRDX II) protein plays a vital role in early embryonic development. This study assessed the beneficial effects of exogenous PRDX II on bovine embryo development at the cellular and molecular levels. To this end, in vitro maturation (IVM) medium was supplemented with various concentrations of PRDX II (0, 6.25, 12.5, 25, 50, and 100μg/mL). Of these, 12.5μg/mL PRDX II was the most effective and significantly promoted embryonic development. Therefore, this concentration of PRDX II was used in subsequent experiments. The percentage of embryos that developed into Day 8 blastocysts and the total number of cells per blastocyst (38.2% and 150.6±5.1) was higher in the PRDX II-treated group than in the control (26.4% and 128.9±3.9, respectively). Moreover, the percent of TUNEL positive cells was higher (P<0.05) in the control than in the PRDX II-treated. Furthermore, PRDX II added to the IVM media increased mitochondria content in blastocysts and decreased the intracellular ROS levels in oocytes and blastocysts compared with the control (P<0.05). The expression of genes associated with blastocyst quality (CDX2 and IFNτ), antioxidant activity (SOD2), and mitochondrial activity (TFAM) was higher, whereas the expression of a gene involved in the apoptotic pathway (c-FOS) was lower, in the PRDX II-treated than in the control group. In conclusion, supplementation of IVM medium with PRDX II promotes development to the blastocyst stage and improves blastocyst quality through reducing ROS, enhancing embryonic mitochondrial activity, and modulating development- related target genes expression.

Original languageEnglish
Pages (from-to)172-183
Number of pages12
JournalAnimal Reproduction Science
Volume159
DOIs
StatePublished - 1 Aug 2015

Keywords

  • Apoptosis
  • Blastocyst
  • Bovine
  • Gene expression
  • Mitochondria
  • PRDX II

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