Effect of placenta extract on human fibroblast

This study has reported the effects of placenta extract on human fibroblast in vitro. We initiated this study on the basis of the hypothesis that placenta extract can influence the proliferation of fibroblasts, and their biologic function, for example, to express cell specific gene or to produce their own extracellular matrix. Fibroblasts in monolayer culture with or without placenta extract were collected and analyzed by MTS assay, ECM assays, and RT-PCR. In results, placenta extract stimulated the proliferation of fibroblasts in monolayer culture. The phenotype of fibroblast was well maintained during expansion in monolayers. Fibroblast with placenta extract produced ECM, collagen and the growth factor, bFGF, more than control. Fibroblasts with placenta extract expressed high COL1A1 gene. This study provides that placenta extract is needed to expand fibroblasts during tissue cultivation, to maintain their differentiated phenotype and to promote their function. In conclusion, these results suggest that placenta extract during cultivation play an important role to control cell behaviors. Furthermore, these results provide a biologic basis for bioengineering.