Effect of plant growth regulators and carbon sources on proliferation and shoot formation of PLBs in Dendrobium candidum

Dendrobium candidum Wallich ex Lindley is a traditional Chinese medicine plant and has been widely used for medicinal and ornamental purposes. In this study, several different factors affecting micro propagation of protocorm-like bodies (PLBs) such as basal media, plant growth regulators, and carbon sources. The proliferation PLB derived from seeds was the best in H₃P₄ basal medium containing 0.1 mg · L^-1 NAA and 0.1 mg · L^-1 Kinetin. PLB growth was the best when 10 g · L^-1 sucrose was added to the carbon atoms in the medium. The rate of shoot formation from the propagated PLB was the highest in 1/4 MS or H₁P₂ medium containing 10 g · L ^-1 sucrose, and the shoot length was longer than the others.