Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription

Hyung Ju Lim; Kwangsoo Kim; Minsang Shin; Jae Ho Jeong; Phil Youl Ryu; Hyon E. Choy

doi:10.1007/s12275-015-4681-8

Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription

Hyung Ju Lim, Kwangsoo Kim, Minsang Shin, Jae Ho Jeong, Phil Youl Ryu, Hyon E. Choy

Department of Medicine

Chonnam National University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone-like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.

Original language	English
Pages (from-to)	250-255
Number of pages	6
Journal	Journal of Microbiology
Volume	53
Issue number	4
DOIs	https://doi.org/10.1007/s12275-015-4681-8
State	Published - 1 Apr 2015

Keywords

E. coli
promoter specificity
RNA polymerase
rpoS
σ38

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10.1007/s12275-015-4681-8

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title = "Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription",

abstract = "σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone-like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.",

keywords = "E. coli, promoter specificity, RNA polymerase, rpoS, σ38",

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AU - Lim, Hyung Ju

AU - Kim, Kwangsoo

AU - Shin, Minsang

AU - Jeong, Jae Ho

AU - Ryu, Phil Youl

AU - Choy, Hyon E.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone-like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.

AB - σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone-like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.

KW - E. coli

KW - promoter specificity

KW - RNA polymerase

KW - rpoS

KW - σ38

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EP - 255

JO - Journal of Microbiology

JF - Journal of Microbiology

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Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription

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