Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells

Sun Uk Kim; Ying Hao Han; Tae Hoon Lee; Byung Hwa Hyun; Sang Ho Lee; Dong Seok Lee; Dae Yeul Yu

doi:10.1538/expanim.53.475

Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells

Sun Uk Kim, Ying Hao Han, Tae Hoon Lee, Byung Hwa Hyun, Sang Ho Lee, Dong Seok Lee, Dae Yeul Yu

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.

Original language	English
Pages (from-to)	475-477
Number of pages	3
Journal	Experimental Animals
Volume	53
Issue number	5
DOIs	https://doi.org/10.1538/expanim.53.475
State	Published - 2004

Keywords

C57BL/6 mice
Germ-line chimera
Host embryo

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10.1538/expanim.53.475

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title = "Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells",

abstract = "Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.",

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AU - Kim, Sun Uk

AU - Han, Ying Hao

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AU - Hyun, Byung Hwa

AU - Lee, Sang Ho

AU - Lee, Dong Seok

AU - Yu, Dae Yeul

PY - 2004

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AB - Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.

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Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells

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Keywords

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