Abstract
Forty weanling piglets (5.6±0.5 kg and 26 to 30 d of age) were used in a 28-d experiment to determine the effects of ß-glucan from Paenibacillus polymyxa and L-theanine on growth performance. Piglets were randomly allotted to four groups (n = 10, 2 animals per pen) provided with the basal feed (control), ß-glucan 400 mg/kg feed, L-theanine 80 mg/kg feed or ß-glucan plus 1-theanine (combination of the above-mentioned concentrations). Body weight and feed consumption were recorded during four weeks. Subsequently, the immunomodulatory effects of ß-glucan and L-theanine were investigated for lipopolysaccharide (LPS)-induced cytokine production in vitro and in vivo on day 28. Although there were no significant differences in the growth performances among the treatment groups, ß-glucan plus L-theanine had 5.6% greater ADG (p = 0.074) on day 21 to 28. ß-Glucan alone or plus L-theanine increased interleukin (IL)-10 levels and decreased interferon (IFN)-γ and tumor necrosis factor (TNF)-α levels in cultured medium by LPS treatment (p<0.05). Plasma IL-10 levels were also increased in the piglets fed with ß-glucan alone or plus L-theanine after LPS challenge (25 μg/kg, i.p.), whereas plasma IFN-γ and TNF-∞ levels were decreased (p<0.05). The levels of IFN-γ in piglets fed with ß-glucan plus L-theanine showed the greatest inhibition after LPS challenges. In conclusion, treatment of ß-glucan alone or plus L-theanine might lessen inflammatory responses against Gram-negative bacterial infection via the inhibition of pro-inflammatory cytokine production and enhancement of anti-inflammatory cytokine production. Further studies are needed to determine an optimal concentration of ß-glucan and L-theanine for improved growth performance.
Original language | English |
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Pages (from-to) | 1753-1759 |
Number of pages | 7 |
Journal | Asian-Australasian Journal of Animal Sciences |
Volume | 21 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2008 |
Keywords
- Cytokine
- L-theanine
- Lipopolysaccharide
- Piglets
- ß-Glucan