TY - JOUR
T1 - Effects of Allopurinol and Apocynin on Renal Ischemia-Reperfusion Injury in Rats
AU - Choi, E. K.
AU - Jung, H.
AU - Kwak, K. H.
AU - Yeo, J.
AU - Yi, S. J.
AU - Park, C. Y.
AU - Ryu, T. H.
AU - Jeon, Y. H.
AU - Park, K. M.
AU - Lim, D. G.
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Background This study evaluated the effects of allopurinol (ALP), a xanthine oxidase inhibitor, and apocynin (APC), a NADPH oxidase inhibitor, administered alone or together, on kidney damage caused by renal ischemia-reperfusion (IR) in rats. Methods Thirty rats were randomly assigned to 5 groups. Group 1 was a sham group. Group 2 was the renal IR control group (30-min ischemia followed by 24-h reperfusion). In groups 3 and 4, ALP or APC, respectively, was administered 1 h before the ischemia. In group 5, ALP and APC were co-administered. Blood urea nitrogen (BUN) and serum creatinine (Cr), renal tissue malondialdehyde (MDA) and superoxide dismutase (SOD), and histological changes were evaluated. Results A significant increase in BUN and Cr level, and histological damage was seen in the IR control group, indicating renal injury. Elevated MDA and decreased SOD levels in the IR control group demonstrated that renal damage occurred through oxidative stress. Pretreatment with ALP or APC alone or together prevented IR-induced renal damage. However, there was no significant difference between treatment with a single drug and co-administration of ALP and APC. Conclusions The use of ALP and/or APC before ischemia may be beneficial to ameliorate renal IR injury.
AB - Background This study evaluated the effects of allopurinol (ALP), a xanthine oxidase inhibitor, and apocynin (APC), a NADPH oxidase inhibitor, administered alone or together, on kidney damage caused by renal ischemia-reperfusion (IR) in rats. Methods Thirty rats were randomly assigned to 5 groups. Group 1 was a sham group. Group 2 was the renal IR control group (30-min ischemia followed by 24-h reperfusion). In groups 3 and 4, ALP or APC, respectively, was administered 1 h before the ischemia. In group 5, ALP and APC were co-administered. Blood urea nitrogen (BUN) and serum creatinine (Cr), renal tissue malondialdehyde (MDA) and superoxide dismutase (SOD), and histological changes were evaluated. Results A significant increase in BUN and Cr level, and histological damage was seen in the IR control group, indicating renal injury. Elevated MDA and decreased SOD levels in the IR control group demonstrated that renal damage occurred through oxidative stress. Pretreatment with ALP or APC alone or together prevented IR-induced renal damage. However, there was no significant difference between treatment with a single drug and co-administration of ALP and APC. Conclusions The use of ALP and/or APC before ischemia may be beneficial to ameliorate renal IR injury.
UR - http://www.scopus.com/inward/record.url?scp=84939445680&partnerID=8YFLogxK
U2 - 10.1016/j.transproceed.2015.06.007
DO - 10.1016/j.transproceed.2015.06.007
M3 - Article
C2 - 26293026
AN - SCOPUS:84939445680
SN - 0041-1345
VL - 47
SP - 1633
EP - 1638
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 6
ER -