Effects of calcium ion incorporation on bone healing of Ti6Al4V alloy implants in rabbit tibiae

Jin Woo Park; Kwang Bum Park; Jo Young Suh

doi:10.1016/j.biomaterials.2007.04.007

Effects of calcium ion incorporation on bone healing of Ti6Al4V alloy implants in rabbit tibiae

Jin Woo Park, Kwang Bum Park, Jo Young Suh

Department of Dentistry

Research output: Contribution to journal › Article › peer-review

132 Scopus citations

Abstract

The biocompatibility of calcium ion (Ca)-incorporated Ti6Al4V alloy implants, produced by hydrothermal treatment using a Ca-containing solution, was investigated. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, Auger electron spectroscopy, and stylus profilometry. The viability of MC3T3-E1 cells on Ca-incorporated machined Ti6Al4V surfaces with different oxide thicknesses was compared with that on untreated machined Ti6Al4V surfaces with MTT assay. The osteoconductivity of the Ca-incorporated Ti6Al4V implants was evaluated by removal torque testing and histomorphometric analysis after 6 weeks of implantation in rabbit tibiae. Our results show that hydrothermal treatment with a Ca-containing solution produced a crystalline CaTiO₃ layer on Ti6Al4V surfaces, and calcium ions were gradually incorporated throughout the oxide layer. After immersion in Hank's balanced salt solution, a considerable apatite deposition was observed on all surfaces of the Ca-incorporated samples. Significant increases in cell viability (p<0.001), removal torque forces, and bone-to-implant contact values (p<0.05) were observed for Ca-incorporated Ti6Al4V implants compared with those for untreated Ti6Al4V implants.

Original language	English
Pages (from-to)	3306-3313
Number of pages	8
Journal	Biomaterials
Volume	28
Issue number	22
DOIs	https://doi.org/10.1016/j.biomaterials.2007.04.007
State	Published - Aug 2007

Keywords

Biocompatibility
Calcium
Cell viability
Histomorphometry
Osseointegration
Titanium alloy

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10.1016/j.biomaterials.2007.04.007

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title = "Effects of calcium ion incorporation on bone healing of Ti6Al4V alloy implants in rabbit tibiae",

abstract = "The biocompatibility of calcium ion (Ca)-incorporated Ti6Al4V alloy implants, produced by hydrothermal treatment using a Ca-containing solution, was investigated. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, Auger electron spectroscopy, and stylus profilometry. The viability of MC3T3-E1 cells on Ca-incorporated machined Ti6Al4V surfaces with different oxide thicknesses was compared with that on untreated machined Ti6Al4V surfaces with MTT assay. The osteoconductivity of the Ca-incorporated Ti6Al4V implants was evaluated by removal torque testing and histomorphometric analysis after 6 weeks of implantation in rabbit tibiae. Our results show that hydrothermal treatment with a Ca-containing solution produced a crystalline CaTiO3 layer on Ti6Al4V surfaces, and calcium ions were gradually incorporated throughout the oxide layer. After immersion in Hank's balanced salt solution, a considerable apatite deposition was observed on all surfaces of the Ca-incorporated samples. Significant increases in cell viability (p<0.001), removal torque forces, and bone-to-implant contact values (p<0.05) were observed for Ca-incorporated Ti6Al4V implants compared with those for untreated Ti6Al4V implants.",

keywords = "Biocompatibility, Calcium, Cell viability, Histomorphometry, Osseointegration, Titanium alloy",

author = "Park, {Jin Woo} and Park, {Kwang Bum} and Suh, {Jo Young}",

year = "2007",

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AB - The biocompatibility of calcium ion (Ca)-incorporated Ti6Al4V alloy implants, produced by hydrothermal treatment using a Ca-containing solution, was investigated. The surface characteristics were evaluated by scanning electron microscopy, thin-film X-ray diffractometry, Auger electron spectroscopy, and stylus profilometry. The viability of MC3T3-E1 cells on Ca-incorporated machined Ti6Al4V surfaces with different oxide thicknesses was compared with that on untreated machined Ti6Al4V surfaces with MTT assay. The osteoconductivity of the Ca-incorporated Ti6Al4V implants was evaluated by removal torque testing and histomorphometric analysis after 6 weeks of implantation in rabbit tibiae. Our results show that hydrothermal treatment with a Ca-containing solution produced a crystalline CaTiO3 layer on Ti6Al4V surfaces, and calcium ions were gradually incorporated throughout the oxide layer. After immersion in Hank's balanced salt solution, a considerable apatite deposition was observed on all surfaces of the Ca-incorporated samples. Significant increases in cell viability (p<0.001), removal torque forces, and bone-to-implant contact values (p<0.05) were observed for Ca-incorporated Ti6Al4V implants compared with those for untreated Ti6Al4V implants.

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Effects of calcium ion incorporation on bone healing of Ti6Al4V alloy implants in rabbit tibiae

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