Effects of Ginsenosides on Vanilloid Receptor (VR1) Channels Expressed in Xenopus Oocytes

Se Yeon Jung; Seok Choi; Yoo Seung Ko; Chul Seung Park; Seikwan Oh; Sung Ryong Koh; Uhtaek Oh; Jae Wook Oh; Man Hee Rhee; Seung Yeol Nah

Effects of Ginsenosides on Vanilloid Receptor (VR1) Channels Expressed in Xenopus Oocytes

Se Yeon Jung, Seok Choi, Yoo Seung Ko, Chul Seung Park, Seikwan Oh, Sung Ryong Koh, Uhtaek Oh, Jae Wook Oh, Man Hee Rhee, Seung Yeol Nah

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Ginsenosides, or ginseng saponins, are biologically active ingredients of Panax ginseng. Accumulating evidence suggests that ginsenosides can alleviate pain from injections of noxious chemicals, such as capsaicin [Nah et al. (2000)]. In this study we examined the effects of ginsenoside Rc on the capsaicin-induced inward current in Xenopus oocytes that expresses the vanilloid receptor 1 (VR1). Ginsenoside Rc enhanced the capsaicin-induced inward current in a concentration-dependent and reversible manner, but ginsenoside Rc itself elicited no membrane currents. The VR1 antagonist capsazepine almost completely blocked the inward current that was elicited by capsaicin plus ginsenoside Rc. We also tested the effect of seven other fractionated ginsenosides (i.e., Rb₁, Rb₂, Rd, Re, Rf, Rg₁, and Rg₂) in addition to ginsenoside Rc. We found that six of them significantly enhanced the inward current that is induced by capsaicin with the following order of potency: Rc > Rf > Rg₁ ≈ Rd > Rb₂ > Rb₁. These results show the possibility that the in vivo effect of ginsenosides against capsaicin-induced pain is derived from their modulation of the VR1 channel function.

Original language	English
Pages (from-to)	342-346
Number of pages	5
Journal	Molecules and Cells
Volume	12
Issue number	3
State	Published - 31 Dec 2001

Keywords

Capsaicin
Ginsenosides
Pain
Vanilloid Receptor
Xenopus Oocytes

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title = "Effects of Ginsenosides on Vanilloid Receptor (VR1) Channels Expressed in Xenopus Oocytes",

abstract = "Ginsenosides, or ginseng saponins, are biologically active ingredients of Panax ginseng. Accumulating evidence suggests that ginsenosides can alleviate pain from injections of noxious chemicals, such as capsaicin [Nah et al. (2000)]. In this study we examined the effects of ginsenoside Rc on the capsaicin-induced inward current in Xenopus oocytes that expresses the vanilloid receptor 1 (VR1). Ginsenoside Rc enhanced the capsaicin-induced inward current in a concentration-dependent and reversible manner, but ginsenoside Rc itself elicited no membrane currents. The VR1 antagonist capsazepine almost completely blocked the inward current that was elicited by capsaicin plus ginsenoside Rc. We also tested the effect of seven other fractionated ginsenosides (i.e., Rb1, Rb2, Rd, Re, Rf, Rg1, and Rg2) in addition to ginsenoside Rc. We found that six of them significantly enhanced the inward current that is induced by capsaicin with the following order of potency: Rc > Rf > Rg1 ≈ Rd > Rb2 > Rb1. These results show the possibility that the in vivo effect of ginsenosides against capsaicin-induced pain is derived from their modulation of the VR1 channel function.",

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author = "Jung, {Se Yeon} and Seok Choi and Ko, {Yoo Seung} and Park, {Chul Seung} and Seikwan Oh and Koh, {Sung Ryong} and Uhtaek Oh and Oh, {Jae Wook} and Rhee, {Man Hee} and Nah, {Seung Yeol}",

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AU - Choi, Seok

AU - Ko, Yoo Seung

AU - Park, Chul Seung

AU - Oh, Seikwan

AU - Koh, Sung Ryong

AU - Oh, Uhtaek

AU - Oh, Jae Wook

AU - Rhee, Man Hee

AU - Nah, Seung Yeol

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AB - Ginsenosides, or ginseng saponins, are biologically active ingredients of Panax ginseng. Accumulating evidence suggests that ginsenosides can alleviate pain from injections of noxious chemicals, such as capsaicin [Nah et al. (2000)]. In this study we examined the effects of ginsenoside Rc on the capsaicin-induced inward current in Xenopus oocytes that expresses the vanilloid receptor 1 (VR1). Ginsenoside Rc enhanced the capsaicin-induced inward current in a concentration-dependent and reversible manner, but ginsenoside Rc itself elicited no membrane currents. The VR1 antagonist capsazepine almost completely blocked the inward current that was elicited by capsaicin plus ginsenoside Rc. We also tested the effect of seven other fractionated ginsenosides (i.e., Rb1, Rb2, Rd, Re, Rf, Rg1, and Rg2) in addition to ginsenoside Rc. We found that six of them significantly enhanced the inward current that is induced by capsaicin with the following order of potency: Rc > Rf > Rg1 ≈ Rd > Rb2 > Rb1. These results show the possibility that the in vivo effect of ginsenosides against capsaicin-induced pain is derived from their modulation of the VR1 channel function.

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KW - Ginsenosides

KW - Pain

KW - Vanilloid Receptor

KW - Xenopus Oocytes

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ER -

Effects of Ginsenosides on Vanilloid Receptor (VR1) Channels Expressed in Xenopus Oocytes

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