Effects of naturally occurring compounds on fibril formation and oxidative stress of β-amyloid

Hee Kim, Byeoung Soo Park, Kwang Geun Lee, Yong Choi Cheol, Sik Jang Sung, Young Ho Kim, Sung Eun Lee

Research output: Contribution to journalArticlepeer-review

292 Scopus citations

Abstract

β-Amyloid (βA)-induced oxidative toxicity on neuronal cells is a principal route in Alzheimer's disease (AD), and its toxicity occurs after fibril formation. Inhibitory or promoting effects of naturally occurring compounds on βA fibril formation were evaluated. Among 214 tested compounds, curcuminoids, flavone type flavonoids, and naphthoquinones were shown to be potent inhibitors of βA fibrilization. The addition of the curcuminoids, curcumin, demethoxycurcumin, and bisdemethoxycurcumin strongly inhibited βA fibril formation. Flavonoids such as quercetin, rhamnetin, and fisetin strongly inhibited βA fibril formation. Limonoids, cinnamic acids, and catechins enhanced fibril formation in vitro. Anthothecol possessed the most enhancing activity on fibril formation of the compounds tested. On the other hand, it was found that curcuminoids showed cytotoxicity with the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay and did not protect HT22 murine neuroblastoma cells from βA(25-35) insult. Two flavone type flavonoids, morin and quercetin, exhibited no cytotoxicity and strongly protected HT22 murine neuroblastoma cells from βA(25-35) oxidative attack. Conclusively, morin or quercetin could be a key molecule for the development of therapeutics for AD.

Original languageEnglish
Pages (from-to)8537-8541
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number22
DOIs
StatePublished - 2 Nov 2005

Keywords

  • β-amyloid
  • Alzheimer's disease
  • bA burden assay
  • Curcumioids
  • Flavonoids
  • Ht22 murine neuroblastoma cell

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